[On the occasion of the 20th anniversary of the accident on the Chernobyl NPP: medical consequences in Armenia].

The aim of this work is the analysis of the indices of the health and of the structure of the sicknesses of the inhabitants of Armenia who took part in the liquidation of the consequences of the Chernobyl accident. Also it is the determination of possible dependence of the frequency of diseases for the most widespread classes of sicknesses on the received dose of the irradiation, according to the data of the clinical examination and dispensarysation; and also it is the revelation of the role of other factors influenced on the health indexes.

[Effects of spatial-imagery task performance on the heart rate variability].

Effects of the efficiency of spatial task performance on the autonomic heart control were studied. Dynamic changes in autonomic balance diagnosed on the heart rate variability during maze-model task performance (the type of spatial-imagery activity) showed that the type of heart rate regulation depended on the performance efficiency. The results suggest that in subjects with low performance efficiency, problems in performance are associated with negative emotions that produce the emotional psychogenic stress.

[Treatment of patients with stage I nonseminomal germ cell testicular tumors].

Three different approaches to treatment of non-seminomal germinogenic testicular tumors (NSGTT) of stage I after orchidofuniculectomy: preventive retroperitoneal lymphadenectomy, preventive chemotherapy, expectant treatment. Recurrences, 5-year recurrence-free and overall survivals reached 17.4, 81.

[Determination of the number of spermatozoa per 1 ml of ejaculate in a stained smear of the semen].

Using statistical and morphometric methods, the authors validate a new method for assessing the quantity of spermatozoa per ml of native ejaculate in a fixed and stained smear. The proposed method is regarded as an alternative to the routine technique.

[The effect of local chemical sympathectomy of the rat uterus on its sensitivity to sex hormones].

Chemical sympathectomy of the rat uterus did not alter the main metabolic processes maintaining the uterus weight even though it changed its response to sex steroids. Sensitivity of endo- and myometrium to progesterone significantly decreased thus inducing a relative increase in the estradiol action.

[The prolactin and thyrotropic hormone content of the blood plasma in neostriatal dysfunction].

In patients with parkinsonism, essential tremor, torsion dystonia, choreic hyperkinesis and hemiballism, a significant increase in the prolactin and thyrotropic hormone was revealed. Possible interrelationship between neuroendocrinological and dysfunction of extrapyramidal structures, is discussed.

[Morphofunctional parameters of the thyroid gland in late periods after prolonged stress in rat strains selected for nervous system excitability].

Thyroid morphology, function, and hormonal levels were studied in two rat strains with genetically determined levels of nervous system excitability immediately and in late periods after prolonged stress resulting in development of a neurosis-like state. A high resistance of the linear characteristics of thyroid morphology and function was revealed whose high functional activity was observed in the rats with a low excitability threshold. Two weeks after neurotization cessation morphofunctional status of the thyroid normalized.

[Weak androgens, their importance experimentally and clinically].

Antisera obtained for certain hormones possessed a high specific effect on homologous hormones. Radioimmunological methods were worked out for determination of these hormones in the blood serum of humans and animals. Clinical use of the above antisera for determination of dehydroepiandrosterone and androstenedione in humans revealed than it could be useful to determine dehydroepiandrosterone in some forms of hypogonadism.

[The content of sex steroid receptors and the activity of marker enzymes of estrogenic action in the uterus of androgen-sterile rats].

In rats with preserved ovaries, both in normal and in neonatally androgenized, the activity of enzymes kreatinphosphokinase and peroxidase cannot serve as the marker of the estradiol effect upon the uterus. In ovariectomised animals, the dynamics of activity of these enzymes corresponds to uterotropic responses to estradiol. but not to the dynamics of hormone receptor complexes.

[The role of progesterone and its receptors in the development of the uterotropic reaction in ovariectomized rats].

In intact rats, the progesterone level in cytosolic fractions of various organs did not correspond to the level of the progesterone receptors. In ovariectomised animals receiving progesterone, the hormone accumulation in the uterus was fulfilled without participation of progesterone receptors. Over half of the progesterone in the rat uterine tissues exists in receptor-unbound "free" form or, possibly, in the form of complexes with small molecular weight.

[17 beta hydroxysteroid dehydrogenase and creatine phosphokinase activities and the levels of sex hormones and their receptors in the uterus of patients with fibromyoma or endocrine sterility].

Examination of endometrial and myometrial activity of steroidogenic enzyme markers, 17 beta-hydroxy steroid dehydrogenase (17 beta-HOSD) and creatine phosphokinase (CP), in women with fibromyoma or endocrine infertility has demonstrated that endometrial 17 beta-HOSD activity corresponded to ovarian progesterone secretion rates but did not correlate with cytosolic progesterone secretion rates but did not correlate with cytosolic progesterone receptor levels. The magnitude of uterine effects of endogenous progesterone in the patients with endocrine infertility and fibromyoma may be inferred from (1) hormone ability to induce 17 beta-HOSD, and (2) ability to reduce cytosolic progesterone receptor levels. Neither endometrial nor myometrial CP represents a "pure" marker of estrogenic influence.

[The dependence of estradiol and progesterone reception in the endometrium on its histological structure in women with the syndrome of luteal phase failure].

A study of the level of the sex steroid receptors in the endometrium of women with the syndrome of insufficiency of the luteal phase of the cycle (ILP-syndrome) has shown that disorders of receptor processes affect, first of all, progesterone receptors. An increase in a degree of a delay of endometrial development was accompanied by a rise of the level of progesterone in the endometrium which depended on the estradiol-progesterone ratio in the circulation, detectable on the 20th day of the menstrual cycle. The ILP-syndrome is characterized by the sufficient induction of progesterone receptors at the end of the proliferative--the beginning of the luteal phase of the cycle and by their insufficient inhibition with a low level of progesterone in the middle of the luteal phase.

[The synthesis of estradiol and progesterone receptors in relation to the biological effects of sex hormones in ovariectomized rats].

The concentration of the estradiol and progesterone receptors in cytosol increased within a week after ovariectomy in rats, the nucleic concentration of receptors sharply diminishing. The uterus atrophy following ovariectomy in associated with a reduction of both nucleic and cytosol receptors in the organ. Administration of estradiol (1 mg) restored the organ mass and the character of hormonal reception within a week.

[Endometrial estradiol and progesterone receptors in women with various clinical forms of luteal insufficiency].

Changes in uterine estradiol and progesterone receptor content in patients with luteal insufficiency (the insufficient luteal phase/ILP/ syndrome developing in the presence of tubal and uterine inflammations, hyperandrogenism or insignificantly increased prolactin level were described. The level of steroid receptors is correlated to blood levels of the respective hormones throughout the cycle. It is suggested that changes in sex steroid receptors are secondary to disorders of progesterone and estradiol secretion in cases of the ILP syndrome developing in the presence of uterine and tubal inflammations, and in patients with increased blood prolactin.

[Reception of progesterone and its biological action in the uterus of neonatally androgenized rats].

The relationship of the level of uterine progesterone receptors and plasma concentration of female sex steroids with the biological effect of progesterone (its effect on collagen content and the number of uterine endometrial glands) was studied in neonatally androgenized female rats aged 90-120 days. In these animals the level of progesterone receptors in the uterine cytosol fraction was raised, in the nuclear fraction it was lowered. Plasma concentration values of estradiol and progesterone in such animals did not differ from those of intact female rats.

[Location of enzymes of acetylcholine hydrolysis in smooth muscle cells of the breast and uterus].

The data on distribution of cholinesterase activity in smooth myocytes of the mammary ducts and in the tissue of normal and pathologically altered myometrium are presented. In the myometrium tumorous tissue, as demonstrate hystochemical investigation results, terminals of the nervous fibers are practically absent, while in the norm they are present in the intermuscular plexuses and accompany blood vessels. At the myomic alteration of the uterine smooth musculature, in myocytes a high enzymatic activity of the acetylcholine hydrolysis enzymes is revealed.

[Hormone-dependent characteristics of the myocyte cell surface in the human myoma and myometrium cultured in diffusion chambers].

The normal and the pathologically changed human myometrium was cultured in the diffusion chamber implanted in the subcutaneous cellular tissue of the rat. In the absence of hormonal influence the growth of myometrium culture is only insignificant, while no myoma cell growth was found at all. Estradiol stimulates the growth and development of the myometrium culture and the myoma.

[Morphological characteristics of the uterine reaction of androgenized rats in the neonatal period to the administration of sex steroids].

In female rats subjected to early neonatal androgenization (50 micrograms of testosterone propionate) the uterus at the age of maturity is of normal sizes and mass whereas its hydro-structure is substantially changed: the content of stromal elements is increased and the number of glands in the endometrium is decreased. The endometrium of androgenized female rats is not sensitive to progesterone influence manifested in the absence of the collagenolytic effect of progesterone and the stimulation of uterine gland formation. It may determine stable sterility of neonatally androgenized female rats after restoration of ovulation in them.