Publications by authors named "Arun Panchapakesan"
The study of host-pathogen interaction often requires interrogating the protein-protein interactions and examining post-translational modifications of the proteins. Traditional protein detection strategies are limited in their sensitivity, specificity, and multiplexing capabilities. The Proximity Ligation Assay (PLA), a versatile and powerful molecular technique, can overcome these limitations.
View Article and Find Full Text PDFArticle Synopsis
- The study focuses on expressing functional proteins in alternative hosts to E. coli, highlighting Lactococcus lactis as a promising option due to its lack of endotoxins during purification.
- Researchers optimized the expression of the challenging HIV-1 Tat protein in L. lactis by testing different promoters and varying conditions like pH and glucose.
- The recombinant Tat protein was shown to be functionally active, suggesting potential for future research on expressing other toxic proteins using this system.
Purpose Of Review: We explore the current status of research on HIV-1 subtype-specific variations and their impact on HIV-1 latency. We also briefly address the controversy surrounding the decision-making process governing the ON/OFF states of HIV-1 transcription, specifically focusing on the regulatory elements, the long terminal repeat (LTR), and Tat. Understanding the decision-making process is crucial for developing effective intervention strategies, such as the 'shock-and-kill' approach, to reactivate latent HIV-1.
View Article and Find Full Text PDF