Publications by authors named "Arun Kumar Guha"

A bacterial strain isolated from soil and identified as Enterobacter cloacae had been found to be capable of producing both intra and extracellular beta-D: -galactosidase.The intracellular enzyme was thermostable and its optimum temperature, pH and time for enzyme-substrate reaction were found to be 50 degrees C, 9.0 and 5 min respectively, using ONPG as substrate.

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A lectin was isolated and purified from the culture filtrate of the plant pathogenic fungus Macrophomina phaseolina by a combination of ammonium sulfate precipitation, affinity chromatography on fetuin-Sepharose 4B and ion-exchange chromatography on DEAE-A 50. The lectin designated MPL was homogeneous by PAGE and HPLC and a monomeric protein with a molecular weight of approximately 34 kDa as demonstrated by SDS-PAGE. It is a glycoprotein and agglutinated human erythrocytes regardless of the human blood type.

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