Preparation and physicochemical characterization of different biocomposite films blended with bacterial exopolysaccharide EPS MC-5 and bacteriocin for food packaging applications.

The study aims to evaluate how bacteriocin and extracellular polymeric substances (EPS) can influence the development of active packaging for food. The components might enhance the performance of packaging materials in terms of their physicochemical properties and their effectiveness in preserving food. Bacteriocin and EPS exert a significant effect in blocking the transmission of UV and visible light radiations.

Green synthesis of copper nanoparticles: a promising solution for drug resistance and cancer therapy challenges.

Green synthesis techniques have drawn a lot of interest lately since they are beneficial to the environment and have potential uses in a variety of industries, including biomedicine. Because of their special physicochemical characteristics, copper nanoparticles (CuNPs) have become one of the most interesting options for use in biological applications among nanomaterials. An overview of green synthesis methods for CuNPs is given in this review, along with a discussion of their applications in cancer therapeutics.

Luminescent TiO nanoparticles synthesized from Mollugo Oppositifolia and Trianthema Portulacastrum for dye degradation, antimicrobial and antioxidant properties.

In modern times, nanoparticles have materialized as indispensable things in contemporary medicine, with a variety of uses in clinical, drug and gene conveyance. In the present study, TiO nanoparticles (NPs) prepared from the leaf extracts of Mollugooppositifolia and Trianthema portulacastrum were compared with the chemical TiO-NPs for antibacterial and antioxidant activities and environment-friendly nature through various tools like the UV-visible, X-ray diffraction with the aid of other analytical techniques like HR-TEM, Fourier transform infrared (FT-IR) and photoluminescence spectroscopic techniques. The morphology of green TiO-NPs is found to be spherical, which is supported by HR-TEM images.

Effect of cryopreservation on motility, DNA integrity and gene expression in grey mullet, Mugil cephalus sperm.

This study documents the effect of cryopreservation on motility, DNA integrity, and gene expression in Mugil cephalus sperm. Fresh sperm were cryopreserved using V2 extender (V2E) or 0.3 M glucose, each in combination with one of three cryoprotective agents (CPAs), i.

Recent Advances in the Production of Pharmaceuticals Using Selective Laser Sintering.

Selective laser sintering (SLS) is an additive manufacturing process that has shown promise in the production of medical devices, including hip cups, knee trays, dental crowns, and hearing aids. SLS-based 3D-printed dosage forms have the potential to revolutionise the production of personalised drugs. The ability to manipulate the porosity of printed materials is a particularly exciting aspect of SLS.

Preparation and functional characterization of the bio-composite film based on chitosan/polyvinyl alcohol blended with bacterial exopolysaccharide EPS MC-5 having antioxidant activities.

In this study, the plate casting method was successfully used to prepare biocomposite films containing EPS from probiotic Enterococcus faecium MC-5 in combination with PVA and chitosan. The findings demonstrated that EPS was uniformly distributed in the film matrices and significantly improved the physicochemical properties of the resulting composite films. The development of intermolecular connections between the polymers was detected by high tensile strength and low water vapour transmission rate.

Biomass derived nitrogen functionalized carbon nanodots for nanomolar determination of levofloxacin in pharmaceutical and water samples.

Binder-free and efficient electrochemical sensing of levofloxacin (LF) was successfully developed based on the nitrogen-doped carbon nanodots (NCNDs). The NCNDs were synthesized by hydrothermal carbonation (180°C for 12 h), and the heteroatom was embedded in aqueous solution of ammonia (NH). Spectral and microscopic characteristization techniques were used to analyze the topological, crystallinity, and chemical binding behavior of synthesized biomass functional material.

Effect of cryopreservation on post-thaw motility and viability of Grey mullet, Mugil cephalus sperm (Linnaeus, 1758).

In this study, we optimized a simple method of cryopreservation for Mugil cephalus sperm based on post-thaw motility and viability. A series of experiments were conducted by changing the extender, cryoprotectant and freezing height above the liquid nitrogen (LN) surface. First, we carried out the cryopreservation using the extender V2E and cryoprotective agents (CPAs) namely, propylene glycol (PG), methanol (MeOH), glycerol (GLY), ethylene glycol (EG), dimethylsulfoxide (Me2SO) and dimethylacetamide (DMA) at a final concentration of 5% and 10%.

Optimization and Physicochemical Characterization of Polysaccharide Purified from Sonneratia caseolaris Mangrove Leaves: a Potential Antioxidant and Antibiofilm Agent.

The Box-Behnken design was applied to determine the optimal parameters of the extraction condition by using the response surface methodology (RSM) from the leaves of Sonneratia caseolaris L. The result indicates the best-optimized conditions used for the extraction of polysaccharides at 84.02 °C temperature, 3.

Effects of polysaccharide-based silver and selenium nanoparticles on growth performance, biochemical parameters, and immune response of .

mangrove leaves polysaccharide (AMLP) was used for the synthesis of polysaccharide-based selenium (AMLP-SeNPs) and silver nanoparticles (AMLP-AgNPs). The synthesized nanoparticles were further characterized by UV-Vis, DLS, FT-IR, X-ray diffraction, and HR-TEM analysis. A 60-day (8 weeks) feeding trial experiment was conducted to investigate the effects of AMLP, AMLP-SeNPs, and AMLP-AgNPs dietary supplementation on growth performance parameters, blood parameters, immunological and enzymatic profiles in .

Enhancement of growth, innate immunity, and disease resistance by probiotic Enterococcus faecium MC-5 against Aeromonas hydrophila in Indian major carp Cirrhinus mrigala.

E. faecium MC-5 was isolated and characterized from the gut content of C. carpio and supplemented with a pellet diet at the ratio of 1.

Characterization of potential probiotic bacteria Enterococcus faecium MC-5 isolated from the gut content of Cyprinus carpio specularis.

The goal of this study was to determine the unique characteristics of Enterococcus faecium MC-5, a probiotic bacteria isolated from the intestine of a fish, Cyprinus carpio specularis, collected from Dal Lake in Srinagar, Kashmir, India. For this, the important valuable probiotic attributes, some functional properties, and safety assessments were analyzed in-vitro for the strain MC-5. The strain E.

Functional and molecular characterization of a cold-active lipase from Psychrobacter celer PU3 with potential antibiofilm property.

The lipase gene from Psychrobacter celer PU3 was cloned into pET-28a(+) expression vector and overexpressed in E. coli BL21 (DE3) pLysS cells. The purified Psychrobacter celer lipase (PCL) was characterized as an alkaline active enzyme and has a molecular mass of around 30 kDa.

Cyan color-emitting nitrogen-functionalized carbon nanodots (NFCNDs) from and their catalytic reduction of organic dyes and fluorescent ink applications.

The present study reports the synthesis of nitrogen-functionalized carbon nanodots (NFCNDs) by a low-cost hydrothermal method using the leaf extract of as a novel carbon precursor. The synthesized NFCNDs were characterized by diverse spectroscopic techniques. The optical properties of N-CNDs were analyzed by UV-visible and fluorescence spectroscopic studies.

Green Sources Derived Carbon Dots for Multifaceted Applications.

For the past decade, the Carbon dots (CDs) a tiny sized carbon nanomaterial are typically much attentive due to their outstanding properties. Nature is a fortune of exciting starting materials that provides many inexpensive and renewable resources which have received the topmost attention of researchers because of non-hazardous and eco-friendly nature that can be used to prepare green CDs by top-down and bottom-up synthesis including hydrothermal carbonization, microwave synthesis, and pyrolysis due to its simple synthetic process, speedy reactions and clear-cut end steps. Compared to chemically derived CDs, green CDs are varied by their properties such as less toxicity, high water dispersibility, superior biocompatibility, good photostability, bright fluorescence, and ease of modification.

Physico-chemical characterization of biosurfactant from PU1 and its application in microbial enhance oil recovery.

Biosurfactant-producing bacteria were isolated from transformer oil-contaminated soil. The strain was identified as PU1 based on its molecular characterization by 16S rRNA gene sequencing. The extraction of biosurfactant was done by acid precipitation method using 2 N hydrochloric acid and further purified by silica-gel column chromatography.

Ecofriendly Synthesis of Fluorescent Nitrogen-Doped Carbon Dots from Coccinia grandis and its Efficient Catalytic Application in the Reduction of Methyl Orange.

Facile and fast hydrothermal process for the synthesis of nitrogen doped carbon dots (N-CDs) from Coccinia grandis (C. grandis) extract is discussed here. The morphology of prepared N-CDs was characterized by high-resolution transmission electron microscopy (HR-TEM), energy dispersive X-ray spectroscopy (EDS), and selected area electron diffraction (SAED) method.

Physicochemical and functional characterization of mannan exopolysaccharide from Weissella confusa MD1 with bioactivities.

The present study focused on production, optimization and characterization of exopolysaccharide (EPS) from Weissella confusa MD1. The purified EPS MD1 was also evaluated for in vitro biological activities. The maximum yield of EPS (10.

Hydrothermally Green Synthesized Nitrogen-Doped Carbon Dots from and Their Catalytic Ability in the Detoxification of Textile Effluents.

A facile method for reduction of textile effluents (TEff) using fluorescent nitrogen-doped carbon dots (N-CDs) has been developed and reported here. The synthesis of N-CDs was done by a hydrothermal carbonization method using the aqueous extract of () fruit as a carbon source and aq NH as a nitrogen dopant. Various analytical techniques have been employed for the characterization of N-CDs.

Sulfated polysaccharides of seagrass suppresses tumor necrosis factor-α-induced chemokine interleukin-8 secretion in HT-29 cell line.

Objectives: The present study aims to investigate the anti-oxidant and anti-inflammatory properties of seagrass sulfated polysaccharide on HT-29 cell line.

Subjects And Methods: Monosaccharides composition was identified using liquid chromatography-mass spectrometry (LC-MS) and the functional groups were analyzed using Fourier transform-infrared (FT-IR) spectroscopy. The antioxidant and anti-inflammatory potential of crude extract and purified fractions was investigated .

Anti-inflammatory and Antioxidant Properties of Probiotic Bacterium AN1 and SNR1 in Wistar Albino Rats.

The potent antioxidant probiotic strains AN1 and SNR1 were assessed for anti-inflammatory properties in carrageenan (acute) and complete Freund's adjuvant-induced inflammation (chronic) models in the present study. The two probiotic strains were administered orally along with feed to the Wistar albino male rats as whole cell as well as microencapsulated form. The following experiments were performed to evaluate the anti-inflammatory properties of probiotic strains and the results were observed that the encapsulated and unencapsulated probiotic strains have exhibited statistically significant decrease in paw thickness.

Cloning, overexpression, purification of bacteriocin enterocin-B and structural analysis, interaction determination of enterocin-A, B against pathogenic bacteria and human cancer cells.

In this present study, a gene (ent-B) encoding the bacteriocin enterocin-B was cloned, overexpressed and purified from Enterococcus faecium por1. The molecular weight of the bacteriocin enterocin-B was observed around 7.2 kDa and exhibited antimicrobial activity against several human pathogenic bacteria.

Molecular characterization and application of lipase from Bacillus sp. PU1 and investigation of structural changes based on pH and temperature using MD simulation.

A gene coding lipase from Bacillus sp. PU1 was cloned and expressed in E. coli BL21(DE3) pLysS.

Biological and catalytic applications of green synthesized fluorescent N-doped carbon dots using Hylocereus undatus.

In this work, a simple hydrothermal route for the synthesis of fluorescent nitrogen doped carbon dots (N-CDs) is reported. The Hylocereus undatus (H. undatus) extract and aqueous ammonia are used as carbon and nitrogen source, respectively.