Genetically modified adipose tissue-derived mesenchymal stem cells overexpressing CXCR4 display increased motility, invasiveness, and homing to bone marrow of NOD/SCID mice.

Objective: This study evaluates usefulness of CXCR4 overexpression via retroviral transduction in adipose tissue-derived mesenchymal stem cells (AT-MSCs) as a strategy to increase their migration and engraftment ability.

Materials And Methods: AT-MSCs were isolated from lipoaspirates from human healthy donors with liberase 3. Cells were transduced with retroviral vector carrying either CXCR4 or green fluorescent protein (GFP) complementary DNA, and neo-resistant colonies were selected and used in experiments.

[Type I and II collagens and mast cells expression in the skin lesions from the patients with localized scleroderma].

Morphea en plaques (MP) and atrophoderma Pasini-Pierini (APP) are by most researchers regarded as specific types of localized scleroderma (LS), but their clinical picture is completely different. Fibrosing process is very complicated, and is connected with presence of collagen (I and III type) and extracellulare matrix deposits in the dermis. Probably mast cells also play an important role in this process.