Publications by authors named "Artjom Stepanjuk"
Endometriosis is a chronic hormone-dependent disease characterized by the spread of endometrial cells outside the uterus, which form endometriotic lesions and disrupt the functions of the affected organs. The etiopathogenesis of endometriosis is still unclear, and thus it is important to examine the genes that may contribute to the establishment of endometriotic lesions. The aim of this study was to investigate the expression of new potential candidate gene (), an inhibitor of carboxypeptidases, in endometrium and endometriotic lesions to elucidate its possible role in endometriosis development.
View Article and Find Full Text PDFStudy Question: Can a combination of the focussed protein kinase assays and a wide-scale proteomic screen pinpoint novel, clinically relevant players in decidualization in vitro and in vivo?
Summary Answer: Rho-dependent protein kinase (ROCK) activity is elevated in response to the combined treatment with progesterone and 8-Br-cAMP during in vitro decidualization, mirrored by increase of ROCK2 mRNA and protein levels and the phosphorylation levels of its downstream target Cofilin-1 (CFL1) in secretory versus proliferative endometrium.
What Is Known Already: Decidualization is associated with extensive changes in gene expression profile, proliferation, metabolism and morphology of endometrium, yet only a few underlying molecular pathways have been systematically explored. In vitro decidualization of endometrial stromal cells (ESCs) can be reportedly induced using multiple protocols with variable physiological relevance.
MUC20 expression marks the receptive phase of the human endometrium.
Reprod Biomed Online
November 2019
Article Synopsis
- Researchers wanted to know how a protein called MUC20 changes in different types of cells in the uterus during the menstrual cycle.
- They looked at data from various studies and used tests to see how much MUC20 is present in the cells during two different phases of the cycle.
- They discovered that MUC20 is mostly found in certain cells when the uterus is ready to receive a fertilized egg, suggesting it could be an important marker for understanding fertility.
Article Synopsis
- Human embryonic stem (hES) cells need special conditions, like certain growth factors and a surface to stick to, to stay healthy and keep their ability to turn into different cell types.
- A protein called integrin β1 is super important for hES cells to stick together, move around, and change into other cells, and if it’s blocked, the hES cells can die or lose their special abilities.
- Using a substance called Y-27632 helped the hES cells move better and change into different cell types, showing that integrin β1 is key for their survival and growth in groups.