Autofluorescence from NADH Conformations Associated with Different Metabolic Pathways Monitored Using Nanosecond-Gated Spectroscopy and Spectral Phasor Analysis.

Cellular NADH conformation is increasingly recognized as an endogenous optical biomarker and metabolic indicator. Recently, we reported a real-time approach for tracking metabolism on the basis of the quantification of UV-excited autofluorescence spectrum shape. Here, we use nanosecond-gated spectral acquisition, combined with spectrum-shape quantification, to monitor the long excited-state lifetime autofluorescence (usually associated with protein-bound NADH conformations) separately from the autofluorescence signal as a whole.

Hypertension management guidelines update and research on the importance of blood pressure control.

According to the Centers for Disease Control and Prevention (CDC), more than 65 million adults in America have hypertension (HTN) and fewer than half of those 65 million people have their blood pressure (BP) under control. More than 1,000 deaths occur each day related to HTN and Americans are at an increased risk of complications related to uncontrolled BP such as heart failure, stroke, myocardial infarction, and kidney disease (). Data from the National Home and Hospice Survey, which is conducted by the CDC, indicate that 41% of home care patients have HTN ().