Use of an Insulation Layer on the Connection Tracks of a Biosensor with Coplanar Electrodes to Increase the Normalized Impedance Variation.

New technologies, such as biosensors and lab-on-a-chip, are reducing time consumption and costs for the detection and characterization of biological cells. One challenge is to detect and characterize cells and bacteria one by one or at a very low concentration. In this case, measurements have very low variations that can be difficult to detect.

Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance.

This paper proposes a simple approach to optimize the operating frequency band of a lab-on-a-chip based on bio-impedance cytometry for a single cell. It mainly concerns applications in low-conductivity media. Bio-impedance allows for the characterization of low cell concentration or single cells by providing an electrical signature.

Influence of Electrode Connection Tracks on Biological Cell Measurements by Impedance Spectroscopy.

The limit of detection of a biological sensor is an important parameter because, when it is optimized, it allows the detection of a reduced number of biological cells and the reduction of the detection time. This parameter can be improved upon with a reduction in electrode size, but the rate of detection is similarly reduced as well. To avoid this problem, we propose a sensor matrix composed of 20 × 20 µm² coplanar square electrodes with a standard clean room manufacturing process.