New 5-Hydroxycoumarin-Based Tyrosyl-DNA Phosphodiesterase I Inhibitors Sensitize Tumor Cell Line to Topotecan.

Tyrosyl-DNA-phosphodiesterase 1 (TDP1) is an important enzyme in the DNA repair system. The ability of the enzyme to repair DNA damage induced by a topoisomerase 1 poison such as the anticancer drug topotecan makes TDP1 a promising target for complex antitumor therapy. In this work, a set of new 5-hydroxycoumarin derivatives containing monoterpene moieties was synthesized.

Deposition of Thick SiO Coatings to Carbonyl Iron Microparticles for Thermal Stability and Microwave Performance.

Thick dielectric SiO shells on the surface of iron particles enhance the thermal and electrodynamic parameters of the iron. A technique to deposit thick, 500-nm, SiO shell to the surface of carbonyl iron (CI) particles was developed. The method consists of repeated deposition of SiO particles with air drying between iterations.

The role of autophagy in the regulation of neuroinflammation in acute ischemic stroke (review of literature).

Postischemic neuroinflammation is a critical pathophysiological process within the entire scheme of cerebral ischemia, covering early damage and the period of tissue repair. It is characterized by microglial and astroglial activation with increased expression of inflammatory mediators and is accompanied by impaired innate and adaptive immune responses. In acute ischemic stroke (IS), neuroinflammation is caused by the response of resident immune cells of microglia and peripheral immunocompetent cells infiltrating the brain tissue, which penetrate the blood-brain barrier (BBB) into the lesion.

The Size Dependence of Microwave Permeability of Hollow Iron Particles.

Hollow ferromagnetic powders of iron were obtained by means of ultrasonic spray pyrolysis. A variation in the conditions of the synthesis allows for the adjustment of the mean size of the hollow iron particles. Iron powders were obtained by this technique, starting from the aqueous solution of iron nitrate of two different concentrations: 10 and 20 wt.

[Are hybrid technologies appropriate for revascularization of aortoiliac-femoral segment?].

Objective: To compare various approaches to revascularization of aortoiliac-femoral segment.

Material And Methods: Data were collected prospectively for retrospective analysis. There were 192 patients with atherosclerotic lesion of the aortoiliac-femoral segment who underwent reconstructive surgeries.

[Apoptosis and autophagy in the pathogenesis of acute ischemic stroke (review of literature).].

According to the World Health Organization, about 5 million people die every year from cerebrovascular disease. At the same time, the proportion of cerebral infarction, or ischemic stroke (IS), among forms of acute cerebrovascular accident reaches 80-85%. Despite the active study of biochemical and morphological changes leading to acute cerebrovascular ischemia, the problem of early diagnosis, prevention, as well as predicting the outcome of this disease is still relevant.

[Choice of the revascularization method for patients of elderly age with infraeinguvinal arterial disease.].

The aim of the study was to perform a comparative evaluation of the use of various methods of reconstructive assistance in the defeat of the femoral-tibial segment in elderly patients with peripheral arterial disease. 253 patients over 60 years old (60-74 years and more than 75 years) with atherosclerotic lesions of arteries below the inguinal ligament were examined and revascularized. According to the type of reconstruction performed, the patients were divided into 3 groups: 98 patients underwent open surgical interventions; 116 patients underwent endovascular interventions; 39 people performed hybrid reconstructions.

[Recurring secondary hyperparathyroidism: case report].

The authors report a rare case of persistently recurring secondary hyperparathyroidism concomitant with chronic renal insufficiency. The patient underwent multiple surgical interventions (parathyroidectomy, implantation of parathyroid autograft into a forearm muscle, removal of hyperplastic autograft tissue). Results of clinical, laboratory, instrumental, and morphological studies are presented.

[New mutation in the CYP17 gene: description of a case].

Deficiency of 17α-hydroxylase is a rare variant of congenital adrenal cortical dysfunction. The defect was first described by E. Biglieri et al.

[Clinical guidelines developed by the Russian Association of Endocrinologists for the diagnosis and treatment of nodular goiter].

The guidelines presented do not pretend to be a systematic presentation of all aspects of the diagnosis and treatment of nodular goiter and are not intended to replace guidelines for various medical disciplines. In real clinical practice, situations may arise that are beyond the scope of the recommendations presented, in connection with which the final decision regarding a specific patient and responsibility for him lies with the attending physician.The recommendations presented are mainly devoted to the diagnosis and treatment of nodular (multinodular) euthyroid colloid, differently proliferating goiter in adults (over 18 years of age) and are the agreed opinion of the RAE experts who developed them.

[Target thin-needle puncture biopsy in diagnosis of thyroid cancer].

One hundred medical histories of patients with thyroid cancer (TC) were analyzed. Patients were divided into two groups depending on the variant of thin-needle biopsy technique (TNB). In group 1TNB was performed with "blind" method (with palpation control)--43 cases, in group 2 target TNB (ultrasound-guided) was carried out--57 cases.

[Long-term results of the surgical treatment of patients with multinodular euthyroid goiter].

Long-term results of treatment of 280 patients operated for multinodal euthyroid goiter (MNEG) in surgical department of Endocrinology Research Center RAMS from 1983 to 1997 were studied. Hormonal examination of peripheral blood, ultrasonic examination of thyroid gland (TG) and zones of regional lymph outflow, thin-needle punch biopsy were used as special methods of investigation. Correlation between recurrence and histological form was studied.

[The hypothalamo-hypophyseal system in patients with multinodular colloid euthyroid goiter].

Eighty patients with multinodular colloidal euthyroid goiter were examined. Thyrotropin-releasing hormone (TRH) test was carried out in 22 patients and 7 healthy women. The results of the test indicate a clear-cut tendency to reduction of hypophyseal TTH reserve in patients with multinodular euthyroid goiter with enlarged thyroid.

[The efficacy of replacement therapy with L-thyroxine in manifest and latent forms of hypothyroidism].

Individual sensitivity of the hypothalamo-hypophyseal-thyroid system was studied in 12 patients with various degrees of euthyroid hyperplasia in the first 2 days of thyroxine therapy and during prolonged therapy. The effectiveness of thyroxine substitution therapy was compared with that of therapy with thyroid drugs in 55 patients with hypothyroidism. Euthyroid hyperplasia of III degree was accompanied by a more marked decrease in the level of TSH than in II degree, however during 3 mos.

[The migration activity of blood leukocytes as an index of the immunologic status of a kidney allograft recipient].

A total of 48 patients with chronic renal failure, 42 of them after allotransplantation of cadaveric kidney, were studied for leukocyte spontaneous migration and corresponding alterations in migration activity in the presence of renal antigens reflecting recipient sensitivity towards organospecific renal antigens. High levels of leukocyte spontaneous migrations and renal antigen sensitization were recorded in patients with acute course of renal transplant rejection versus those with no evidence of rejection or the persons with slowly progressing chronic rejection. It was stated that alterations in leukocyte migratory activity, both spontaneous and challenged by presence of renal antigen, could demonstrate the activity of immune processes in a recipient of renal transplant.

[Effect of anti-brain sera on the appearance of hypersensitivity of the delayed type and its adoptive transfer].

The effect of rabbit immunosera against CBA mouse brain on lymphoid cells of guinea pigs was studied in experiments with adoptive transfer of hypersensitivity of the delayed type. In vitro treatment of the transferred cells abolished their capacity for adoptive transfer. Such an effect of the sera was not selective with respect to the sensitizing antigen, i.

[So-called antireceptor sera].

Experiments in delayed type hypersensitivity transfer were carried out with the aim of studying the ability of rabbit antisera against peritoneal exudate cells of rats sensitized with bovine gamma globulin or rabbit kidney tissue antigen to block peritoneal exudate cells of guinea pigs. In the serological test the antisera prepared against the cells of sensitized rats and tentatively named "receptor antisera", reacted not only with the cells of these rats, respectively, but also with guinea pig cells. In hypersensitivity transfer experiments in guinea pigs receptor antisera showed a blocking effect on the transferred cells, making them incapable of transferring hypersensitivity, i.

[Further study of the problem of specific cellular receptors in delayed hypersensitivity].

The work presents the materials obtained as a result of the further study of specific T lymphocyte receptors with the use of so-called receptor antisera, i. e. antisera against the lymphoid cells of mice sensitized with one of the two antigens (Macobacterium tuberculosis or bovine gamma globulin); thus these differed from control antisera against the lymphoid cells of intact mice.

[Study of specific cellular receptors in delayed type hypersensitivity].

The effect of immunosera (rabbit) against the lymphoid cells of mice sensitized with antigens causing development of hypersensitivity of delayed type (tuberculosis bacillus, tissue antigen from rabbit kidney) on the specific activity of lymphoid cells of guinea pigs was tested. The mentioned sera blocked the capacity of lymphoid cells of guinea pigs to transfer the state of sensitization from the sensitized donors to the intact recipients. Blocking was strictly specific and this capacity was absent in the immunosera against the normal lymphoid cells.