Publications by authors named "Arsenij Kokorin"
Multi-enzyme cascades enable the production of valuable chemical compounds, and fusion of the enzymes that catalyze these reactions can improve the reaction outcome. In this work, P450 BM3 from Bacillus megaterium and an alcohol dehydrogenase from Sphingomonas yanoikuyae were fused to bifunctional constructs to enable cofactor regeneration and improve the in vitro two-step oxidation of (+)-valencene to (+)-nootkatone. An up to 1.
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- Researchers fused genes from cytochrome P450 BM3 and formate dehydrogenase to create multifunctional enzymes aimed at improving stability and activity.
- The study varied the arrangement of the genes and linkers and compared the fused enzymes with individual ones in terms of substrate conversion and stability.
- A significant increase in activity (up to threefold) was noted for the fusion constructs, indicating that the fusion may cause conformational changes in P450 BM3, although no NADPH channeling was observed.
Ribosome-inactivating proteins (RIPs) are enzymes with N-glycosylase activity that remove adenine bases from the ribosomal RNA. In theory, one single RIP molecule internalized into a cell is sufficient to induce cell death. For this reason, RIPs are of high potential as toxic payload for anti-tumor therapy.
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