The residues Leu(Ile)475-Ile(Leu, Val, Ala)476, contained in the extended carboxyl cytoplasmic tail, are critical for targeting of the resident lysosomal membrane protein LIMP II to lysosomes.

LIMP II, a type II lysosomal integral membrane protein, and the CD36/LIMP II construct are targeted to lysosomes by means of a signal expressed in the tyrosine-lacking carboxyl cytoplasmic tail of LIMP II (Vega, M. A., Rodriguez, F.

Identification and partial characterization of an immunoglobulin A protease associated with human sperm.

The ability of human sperm to evade local humoral immune response at the female genital tract has received little attention. As some microorganisms inactivate secretory immunoglobulin A (S-IgA) by enzymatic degradation as a part of their pathogenic mechanism, we decided to test the hypothesis that there is an IgA protease in human semen that helps sperm to progress through the cervix uteri. Incubation of different semen fractions in the presence of S-IgA revealed IgA protease activity associated with raw semen or sperm but not with seminal plasma; raw semen and sperm produced two split products of 66 kDa and 45 kDa, respectively, in SDS-PAGE.

[Hepatitis C during puerperium].

Screening of donor blood for hepatitis B led to recognition that most cases of post-transfusion hepatitis were due to an agent known as non-A, non-B. Until recently the causative agent for non-A, non-B hepatitis was identified, being denominated hepatitis C virus. It is estimated that one-half of patient with hepatitis C develop chronic liver disease, and 20% of this will develop cirrhosis.

Gastric heterotopic mucosa in the rectum with Helicobacter pylori-like organisms: a rare cause of rectal bleeding.

We report the case of a 9-year-old boy who presented with chronic rectal bleeding. On proctosigmoidoscopy, a polypoid elevation in the rectal ampulla measuring 5 x 3 cm was found. Biopsies were reported to show gastric mucosa.

Anopheles vestitipennis, the probable vector of Plasmodium vivax in the Lacandon forest of Chiapas, México.

Baseline studies to evaluate the role of different anopheline mosquitoes as probable vectors of malaria in the Lacandon rainforest region in Chiapas (México) were carried out in 3 rural villages between June and November 1988. Anopheles vestitipennis was the most abundant species in all the villages, followed by An. albimanus, An.

Effect of indoor residual spraying of DDT and bendiocarb on the feeding patterns of Anopheles pseudopunctipennis in Mexico.

Intense and persistent use of DDT for malaria control has increased resistance and induced exophilic behavior of Anopheles pseudopunctipennis. An evaluation of bendiocarb and DDT to control this species in Sinaloa, Mexico, showed that, in spite of DDT-resistance, both insecticides produced similar effects. Feeding patterns were analyzed to explain these results.

Identification of a region of Escherichia coli ribosomal protein L2 required for the assembly of L16 into the 50 S ribosomal subunit.

In vitro mutagenesis of rplB was used to generate changes in a conserved region of Escherichia coli ribosomal protein L2 between Gly221 and His231. Mutants were selected by temperature sensitivity using an inducible expression system. A mutant L2 protein with the deletion of Thr222 to Asp228 was readily distinguishable from wild-type L2 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and ribosomes from the strain overexpressing this mutant protein were characterized by sucrose density gradient centrifugation and protein composition.

Vascular dementia. A clinicopathological study.

We have reviewed the clinical and pathological records of 40 aged patients who showed only vascular lesions on histological examination. They were followed up for 3.5 +/- 6.

Results of treatment of uncomplicated urogenital gonorrhoea with enoxacin compared with ceftriaxone.

The increasing incidence of penicillinase producing Neissreria gonorrhoeae (PPNG) and chromosomally-mediated resistant N. gonorrhoeae (CMRNG) has generated a need for therapy other than penicillin. PPNG constitutes about 30% of the circulating strains of gonococci in the high risk population in Mexico City.

[Complications of chickenpox].

A patient with typical varicella (chickenpox) developed several complications: nephrotic syndrome following glomerulonephritis, trombocytopenic purpura, gastrointestinal bleeding, disseminated intravascular coagulation, encephalitis and bronchopneumonia. The course was satisfactory having been given antibiotics, heparin and peritoneal dialysis. Recovery was 100%.

[Treatment of chronic osteomyelitis].

Levels of Cephaloridine and Cefazoline were measured in serum, pus and bone in 20 patients with chronic osteomyelitis and the efficiency of these drugs in the treatment of osteomyelitis was evaluated.

[Pleuropulmonary lesions due to Haemophilus influenzae].

Empyema due to Hemophilus influenzae has been reported only rarely. This report describes the cases of ten children with empyema due to this organism. Diagnosis was made through growth of the organism in pure culture from pleural effusion.