An insight into transcriptome of Cyathus bulleri for lignocellulase expression on wheat bran.

To identify enzymes that can be effectively used for hydrolysis of lignocellulosic biomass, an attractive carbon source in biorefineries, transcriptome analysis was carried out of wheat bran grown fungus, Cyathus bulleri. A comprehensive set of transcripts, encoding carbohydrate active enzymes, were identified. These belonged to 55, 32, 12, 11 and 7 different families of the enzyme classes of Glycoside Hydrolases (GHs), Glycosyl Transferases (GTs), Auxiliary Activities (AAs), Carbohydrate Esterases (CEs) and Polysaccharide Lyases (PLs) respectively.

Identification and evaluation of bioremediation potential of laccase isoforms produced by Cyathus bulleri on wheat bran.

Multiplicity in laccases among lignin degrading fungal species is of interest as it confers the ability to degrade several types of lignocellulosics. The combination of laccases produced on such substrates could be beneficial for treatment of complex aromatics, including dyes. In this study, we report on production of high units (679.

Decolorization of complex dyes and textile effluent by extracellular enzymes of Cyathus bulleri cultivated on agro-residues/domestic wastes and proposed pathway of degradation of Kiton blue A and reactive orange 16.

In this study, the white-rot fungus Cyathus bulleri was cultivated on low-cost agro-residues, namely wheat bran (WB), wheat straw (WS), and domestic waste orange peel (OP) for production of ligninolytic enzymes. Of the three substrates, WB and OP served as good materials for the production of laccase with no requirement of additional carbon or nitrogen source. Specific laccase activity of 94.