Nanoval Technology-An Intermediate Process between Meltblown and Spunbond.

The idea of "Nanoval technology" origins in the metal injection molding for gas atomization of metal powders and the knowledge of spunbond technologies for the creation of thermoplastic nonwovens using the benefits of both techniques. In this study, we evaluated processing limits experimentally for the spinning of different types of polypropylene, further standard polymers, and polyphenylene sulfide, marked by defect-free fiber creation. A numerical simulation study of the turbulent air flow as well as filament motion in the process visualized that the turnover from uniaxial flow (initial stretching caused by the high air velocity directed at the spinning die) to turbulent viscoelastic behavior occurs significantly earlier than in the melt-blown process.

Fluorescence signatures of SARS-CoV-2 spike S1 proteins and a human ACE-2: excitation-emission maps and fluorescence lifetimes.

Significance: Fast and reliable detection of infectious SARS-CoV-2 virus loads is an important issue. Fluorescence spectroscopy is a sensitive tool to do so in clean environments. This presumes a comprehensive knowledge of fluorescence data.

Multispectral LIF-Based Standoff Detection System for the Classification of CBE Hazards by Spectral and Temporal Features.

Laser-induced fluorescence (LIF) is a well-established technique for monitoring chemical processes and for the standoff detection of biological substances because of its simple technical implementation and high sensitivity. Frequently, standoff LIF spectra from large molecules and bio-agents are only slightly structured and a gain of deeper information, such as classification, let alone identification, might become challenging. Improving the LIF technology by recording spectral and additionally time-resolved fluorescence emission, a significant gain of information can be achieved.

Primary light-induced reaction steps of reversibly photoswitchable fluorescent protein Padron0.9 investigated by femtosecond spectroscopy.

The reversible photoswitching of the photochromic fluorescent protein Padron0.9 involves a cis-trans isomerization of the chromophore. Both isomers are subjected to a protonation equilibrium between a neutral and a deprotonated form.

Nanoscopy in a living multicellular organism expressing GFP.

We report superresolution fluorescence microscopy in an intact living organism, namely Caenorhabditis elegans nematodes expressing green fluorescent protein (GFP)-fusion proteins. We also superresolve, by stimulated emission depletion (STED) microscopy, living cultured cells, demonstrating that STED microscopy with GFP can be widely applied. STED with GFP can be performed with both pulsed and continuous-wave lasers spanning a wide wavelength range from at least 556-592 nm.