On-tissue dataset-dependent MALDI-TIMS-MS bioimaging.

Trapped ion mobility spectrometry (TIMS) adds an additional separation dimension to mass spectrometry (MS) imaging, however, the lack of fragmentation spectra (MS) impedes confident compound annotation in spatial metabolomics. Here, we describe spatial ion mobility-scheduled exhaustive fragmentation (SIMSEF), a dataset-dependent acquisition strategy that augments TIMS-MS imaging datasets with MS spectra. The fragmentation experiments are systematically distributed across the sample and scheduled for multiple collision energies per precursor ion.

Laser desorption/ionization-mass spectrometry for the analysis of interphases in lithium ion batteries.

Laser desorption/ionization-mass spectrometry (LDI-MS) is introduced as a complementary technique for the analysis of interphases formed at electrode|electrolyte interfaces in lithium ion batteries (LIBs). An understanding of these interphases is crucial for designing interphase-forming electrolyte formulations and increasing battery lifetime. Especially organic species are analyzed more effectively using LDI-MS than with established methodologies.

Mobility-resolved broadband dissociation and parallel reaction monitoring for laser desorption/ionization-mass spectrometry - Tattoo pigment identification supported by trapped ion mobility spectrometry.

In this work, trapped ion mobility spectrometry (TIMS) was introduced to facilitate tandem mass spectrometry (MS) experiments for laser desorption/ionization-mass spectrometry (LDI-MS) as mobility-resolved fragmentation. The mobility separation of desorbed ions was followed by subsequent fragmentation using data-independent broadband collision-induced dissociation (bbCID) or targeted fragmentation through a prototypic version of parallel reaction monitoring-parallel accumulation serial fragmentation (prm-PASEF) for LDI. Both mobility-resolved fragmentation options, TIMS-bbCID and prm-PASEF, were applied to LDI point measurements to identify organic pigments in tattoo inks.

Rotationally tunable multi-focal diffractive moiré lenses.

In this work, we show how the combination of cascaded multi-value phase diffractive optical elements can form a multi-focal moiré zone plate with tunable optical power in each diffraction order. The rotationally tunable moiré zone plate is capable of generating an array of equal intensity focal spots with a precisely adjustable axial distance along the propagation direction. Numerical simulations as well as experimental results verify that multiple focal spots are generated, and the distance between the generated uniform foci can be adjusted by a mutual rotation of one multi-value phase diffractive element with respect to the other.

Husimi functions for coupled optical resonators.

Phase-space analysis has been widely used in the past for the study of optical resonant systems. While it is usually employed to analyze the far-field behavior of resonant systems, we focus here on its applicability to coupling problems. By looking at the phase-space description of both the resonant mode and the exciting source, it is possible to understand the coupling mechanisms as well as to gain insights and approximate the coupling behavior with reduced computational effort.

Mass-Spectrometric Imaging of Electrode Surfaces-a View on Electrochemical Side Reactions.

Electrochemical side reactions, often referred to as "electrode fouling", are known to be a major challenge in electro-organic synthesis and the functionality of modern batteries. Often, polymerization of one or more components is observed. When reaching their limit of solubility, those polymers tend to adsorb on the surface of the electrode, resulting in a passivation of the respective electrode area, which may impact electrochemical performance.

Complementing Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry Imaging with Chromatography Data for Improved Assignment of Isobaric and Isomeric Phospholipids Utilizing Trapped Ion Mobility-Mass Spectrometry.

Lipids, such for example the multifaceted category of glycerophospholipids (GP), play a major role in many biological processes. High-resolution mass spectrometry is able to identify these highly diverse lipid species in combination with fragmentation experiments (MS/MS) on the basis of the accurate / and fragmentation pattern. However, for the differentiation of isomeric lipids or isobaric interferences, more elaborate separation methods are required.

Automated Chiral Analysis of Amino Acids Based on Chiral Derivatization and Trapped Ion Mobility-Mass Spectrometry.

A fast and fully automated method for chiral analysis has been developed by combining a chiral derivatization approach with high-resolution trapped ion mobility separation. Although the presented approach can be potentially applied to diverse types of chiral compounds, several benchmark amino acids were used as model compounds, focusing on the smallest amino acid alanine. An autosampler with an integrated chromatography system was used for inline chiral derivatization with ()-naproxen chloride and subsequent preseparation.

Fabrication and characterization of deformed microdisk cavities in silicon dioxide with high Q-factor.

We demonstrate the excitation and characterization of whispering gallery modes in a deformed optical microcavity. To fabricate deformed microdisk microresonators we established a fabrication process relying on dry plasma etching tools for many degrees of freedom and a shape-accurate morphology. This approach allowed us to fabricate resonators of different sizes with a controlled sidewall angle and underetching in large quantities with reproducible properties such as a surface roughness ≤2.

Hydroperoxylated vs Dihydroxylated Lipids: Differentiation of Isomeric Cardiolipin Oxidation Products by Multidimensional Separation Techniques.

In recent years, cardiolipin (CL) oxidation products were recognized as potential markers for mitochondrial dysfunction in conjunction with age related diseases. The analysis of oxidized CL requires powerful analysis techniques due to high structural diversity. In addition, low concentrations of partly labile compounds pose a special challenge, supplemented by the occurrence of isomeric compounds, e.