Efficacy of ivermectin pour-on against Ostertagia ostertagi infection and residues in the American bison, Bison bison.

Sixteen American bison, Bison bison, were artificially infected with 10(5) infective stage larvae of Ostertagia ostertagi on 21 April 1993. At 42 days post-infection eight bison were treated with 0.5% ivermectin pour-on (500 micrograms/kg bodyweight) and eight treated with the carrier only.

Reduced, positive nitrogen balance and elevated plasma free fatty acid concentration in growing, glutamate-induced obese rats.

Glutamate-induced obesity of Wistar-rats is known to develop under normophagic and normoinsulinemic conditions, although hyperphagia and hyperinsulinemia are common to obese individuals. Rats of this obesity model show retarded growth, reduced mass of some organs, carcass and whole body as well as an extraordinary high fat content, whereas protein content is reduced. In this study, nitrogen (N) balance, urinary excretion of urea-N, ammonia-N, creatine-N and alpha-amino acid-N and plasma free fatty acid concentration of growing, glutamate-induced obese rats were determined.

Further characterization of Pasteurella haemolytica-like bacteria isolated from swine enteritis.

DNA-DNA hybridization studies were conducted on six Pasteurella haemolytica-like (PHL) organisms recovered from cases of swine enteritis. Chromosomal-enriched fractions of PHL organisms served as the source of DNA for Southern blots or as whole-chromosomal DNA probes. Under stringent hybridization conditions, chromosomal DNA probes of a prototype PHL (strain 6213A) organism distinguished other PHL organisms from Pasteurella haemolytica types A1 and T3, Pasteurella multiocida types A:1 and A:3, Escherichia coli, Pseudomonas aeruginosa, Actinobacillus pleuropneumoniae type 1, and Salmonella cholerasuis.

In vivo assessment of insulin binding in different organs of growing and adult glutamate-induced obese rats.

Injection of Na-L-glutamate into neonate Wistar-rats (2 mg/g body mass s.c.; day 1-5 of life) induces hypothalamic lesions, which are followed by hypoplastic-hypertrophic obesity despite normophagia.

Examination of brown adipose tissue thermogenesis of glutamate-induced obese rats by differential temperature measurements.

Differential temperature measurement between interscapular brown adipose tissue (BAT, Tbat), rectum (Trect) and a subcutaneous point in the back left of the vertebral column (Tsc) was useful for examination of BAT-thermogenesis in glutamate-induced obese Wistar-rats. Positive temperature gradients Tbat-Tsc pointed to a basal BAT-thermogenesis, whereas negative temperature gradients Tbat-Trect did not indicate that heat production in lean and obese rats. One may conclude from this, that inclusion of subcutaneous points outside the BAT improves sensitivity of differential temperature measurements for BAT-thermogenesis.

Assessment of cold induced alterations in catecholamine turnover of lean and glutamate-treated obese rats.

Excretion of norepinephrine (NE) and vanillylmandelic acid (VMA) in urine as well as NE-turnover in tissues from lean and glutamate-treated obese rats were determined in warm and cold environment. NE-and VMA-excretion in urine was elevated by cold exposure, indicating an activation of the sympathetic nervous system in animals of both groups. Organspecific NE-turnover responds with higher sensitivity to cold in obese rats but without complete compensation in brown adipose tissue.

Recombinant DNA probe detecting Eperythrozoon suis in swine blood.

A genomic library to Eperythrozoon suis DNA was constructed in lambda gt11, and from this library, E suis clone KSU-2 was identified as a potential diagnostic probe. In hybridization experiments that used 100-microliters samples of blood collected in chaotropic salt solutions, the KSU-2 probe hybridized strongly with purified E suis organisms and blood samples from splenectomized swine that were parasitized with E suis. However, the probe under stringent conditions did not give radiographic indications of hybridizing with equine blood DNA, bovine blood DNA infected with Anaplasma marginale, canine blood DNA infected with Ehrlichia canis, feline blood DNA infected with Haemobartonella felis, or uninfected swine blood DNA.

Echinococcal cyst causing bladder outlet obstruction. A case report.

The case of a 36-year-old black man with bladder outlet obstruction caused by a retrovesical echinococcal cyst is described. The diagnosis was made by conventional radiography, pelvic ultrasonography and computed tomography. The cyst was removed and the patient made an uneventful recovery.

Fournier's gangrene: necrotising fasciitis of the male genitalia.

The aetiology, clinical syndrome and outcome of nine patients with necrotising subcutaneous infections of their external genitalia have been analysed. These patients constituted just under 1% of all of our urological admissions during a nine-month period. Eight of the nine patients suffered from the secondary variety of this disease with urethral strictures and extravasation being the most common precipitating conditions.

Tumors in fish caught in polluted waters: possible explanations.

The largest excess in tumor incidence due to pollution (616%) was observed in the fish species: Ictalurus nebulosis (the brown bullhead). Extensive analysis of waters on the Fox River in Illinois disclosed a heavy chemical and physical organic and inorganic materials far exceeding State and Federal minimal safety standards. Additional tests showed the presence of numerous human viral agents in the Fox River.