Publications by authors named "Arnaud Reignier"

Purpose: To evaluate ovarian stimulation response in couples undergoing preimplantation genetic testing (PGT-M) for myotonic dystrophy type 1 (DM1) METHODS: Retrospective, observational, multicentric study. Parameters of ovarian response and PGT-M outcomes were compared according to the DM1-affected patient (female or male). A total of 229 couples underwent at least one controlled ovarian hyperstimulation cycle for the PGT-M procedure.

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Background: Spinal muscular atrophy (SMA) patients benefit from pre-mRNA splicing modifiers targeting the SMN2 gene, which aims to increase functional SMN production. The animal toxicity affecting spermatogenesis associated with one such treatment raised questions about male SMA patients' spermatogenesis.

Methods: This descriptive, cross-sectional study was conducted from June 2022 to July 2023.

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Study Question: Can machine learning predict the number of oocytes retrieved from controlled ovarian hyperstimulation (COH)?

Summary Answer: Three machine-learning models were successfully trained to predict the number of oocytes retrieved from COH.

What Is Known Already: A number of previous studies have identified and built predictive models on factors that influence the number of oocytes retrieved during COH. Many of these studies are, however, limited in the fact that they only consider a small number of variables in isolation.

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Isolation of individual cells ensures detailed analysis of human embryos and promotes our understanding of molecular mechanisms driving embryo development and cell specification. Here, we present a protocol for the processing of human embryos for single-cell analysis. We describe steps for growing embryos and individualizing cells from the polar and the mural parts of trophectoderm at the blastocyst stage using laser dissection.

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Since 2021, assisted reproductive technologies (ART) are available to infertile couples, but also to single women and female couples. The process of in vitro fertilization (IVF) has allowed to cross the threshold of 5 million births worldwide, between 1978 and 2013. However, the failure rate per each IVF cycle is estimated to be around 75%.

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Although the duration of progesterone administration in Hormonal Replacement Therapy (HRT) cycles before frozen embryo transfer is standardized, the optimal duration of oestrogen treatment remains controversial. In this monocentric retrospective study conducted in all single frozen blastocyst transfer (FBT) performed with HRT between January 2016 and July 2019, we evaluated the association between the duration of oestradiol treatment before FBT and live birth rate (LBR) in HRT cycles. Cycles were gathered in 3 groups according to quartiles of duration of oestrogen treatment.

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Article Synopsis
  • The study investigates the impact of assisted reproductive technology (ART) using donor sperm on sexual function in heterosexual couples dealing with infertility, noting a potential connection between infertility and sexual dysfunction.
  • Findings reveal that 39.3% of women and 26.5% of men in the study experienced sexual dysfunction, although no significant differences were found between those with and without sexual dysfunction.
  • The study highlights the need for further research, particularly on psychological interventions, as no specific predictors of sexual dysfunction could be identified in the small sample size.
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  • Day 5 fresh blastocyst transfers yield better clinical pregnancy and live birth rates compared to day 6 transfers.
  • The study examined the best approach for slowly developing blastocysts, comparing transfers on day 5 vs. day 6 or freezing them.
  • Results showed that fresh transfers of expanded blastocysts on day 5 had significantly higher success rates, while slowly developing blastocysts can still be transferred fresh on day 5 instead of delaying or freezing.
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  • Understanding how human embryos develop before implantation can enhance reproductive technologies and stem cell research.
  • The study uses advanced techniques like single-cell RNA sequencing to analyze mouse and human embryos' development over time, noting crucial changes in gene expression.
  • Key findings include the timing of different cell types forming and specific markers that indicate development stages, paving the way for better insights into embryonic development.
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  • This study evaluates the effectiveness of time-lapse technology (TLT) in improving the total cumulative live birth rate (TCLBR) and shortening the time to live birth (TTLB) compared to conventional incubation (CI) during IVF treatments.
  • The research involved 151 couples using TLT and 250 couples using CI, tracking their outcomes up to 2020.
  • Results indicated that TLT significantly increased the TCLBR (66.9% vs. 56.4%) and reduced the median time to live birth (464 days vs. 596 days) compared to CI, highlighting its potential benefits in IVF care.
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  • The study investigates the effectiveness of two automated semen analysis systems compared to traditional manual sperm assessment, focusing on male infertility diagnosis.
  • Both automated systems showed no significant differences from manual assessments in most sperm parameters, except for sperm morphology, where one system performed slightly poorer than the other.
  • The findings support the use of these automated systems in andrology labs for routine sperm testing due to their moderate to high correlation with manual assessments.
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  • Anti-Mullerian Hormone (AMH) is a key marker for assessing ovarian reserve but its link to oocyte quality and pregnancy outcomes needs more research.
  • A study compared miscarriage rates in young women (<37 years) with low serum AMH levels indicating diminished ovarian reserve, against those with normal levels after fresh blastocyst transfers.
  • Results showed no significant difference in miscarriage rates (9.5% vs 6.8%) or other reproductive outcomes, indicating low AMH levels do not increase miscarriage risks in this age group.
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  • The study aimed to investigate the relationship between the morphology of blastocysts and maternal first trimester serum markers in IVF pregnancies following fresh single blastocyst transfer.
  • Conducted over six years with 122 women, blastocysts were categorized based on quality and serum markers were assessed between 9 and 11 weeks of gestation.
  • Results indicated no significant differences in first trimester serum markers among blastocyst quality groups, nor between the morphology of the inner cell mass and serum markers.
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  • * Conducted between May 2013 and August 2017, the study involved analyzing late morphokinetic parameters from 1691 biopsied embryos and 2578 non-biopsied embryos cultured in a time-lapse monitoring (TLM) system.
  • * Results showed that late morphokinetic events like compaction and blastulation occurred significantly earlier in the biopsied group, suggesting a potential influence of the biopsy on embryo development that warrants
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  • Embryo vitrification is becoming common in IVF, and artificial shrinkage before this process is believed to enhance blastocyst survival after warming.
  • A systematic review and meta-analysis of eight studies showed that while blastocyst survival and clinical pregnancy rates improve with this technique, implantation and live birth rates did not show any significant difference compared to controls.
  • The study suggests that more rigorous randomized trials are needed to solidify these findings and further evaluate the effectiveness of artificial shrinkage in improving IVF outcomes.
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  • - The study aimed to evaluate the effectiveness of two versions of the KIDScore™ models in predicting implantation and live birth rates post day 5 embryo transfer, addressing the lack of externally validated predictive models in previous research.
  • - Conducted on 210 ICSI cycles using time-lapse imaging, the results showed a 35.7% implantation and live birth rate, with both KIDScore™ models demonstrating a strong correlation with implantation (r=0.96 and r=0.90) but limited predictive power overall (AUC 0.60).
  • - Though the KIDScore™ models are associated with implantation success, their predictive capability needs improvement, emphasizing the importance of embryologists' expertise in making the final embryo
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  • The study investigated whether morphokinetic parameters of embryos could help differentiate between balanced and unbalanced embryos in translocation carriers undergoing preimplantation genetic testing.
  • Significant differences were found in several morphokinetic parameters and abnormal cellular events, but none were reliable enough to predict chromosomal status accurately.
  • The conclusion emphasized that time-lapse imaging should not be used as a diagnostic method for determining chromosomal status in this specific group of patients.
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  • The study aimed to evaluate the use and perception of time-lapse imaging (TLI) systems in IVF laboratories in France, noting that its prevalence may differ from other countries like the USA.
  • An online survey targeted 210 embryologists, with 78 responding, revealing that about 38.5% were using TLI, primarily for unselected patients, while cost was the main barrier for non-users.
  • Despite many embryologists believing TLI is superior to standard methods and improves culture conditions, over half acknowledged a lack of strong evidence supporting its clinical effectiveness.
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Does corifollitropin alfa associated with hp-HMG protocol from the beginning of ovarian stimulation perform better than high dose rFSH alone for ovarian stimulation with GnRH antagonist in poor responders? This retrospective, monocentric, case-control pilot study was conducted in 65 poor responders (Bologna criteria) undergoing 2 consecutive IVF cycles. All patients underwent a first ovarian stimulation cycle with high dose rFSH (≥300 IU/day) alone in antagonist protocol, unfortunately leading to poor ovarian response and no pregnancy. The following cycle was performed with 150 μg of corifollitropin alfa associated with daily injections of hp-HMG from the beginning of the cycle.

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Research Question: Does ovarian reserve and ovarian response to ovarian stimulation in women with cancer undergoing oocyte vitrification for fertility preservation vary according to the type of malignancy?

Design: Retrospective cohort study including 105 women aged between 18 and 40 years, who were referred for fertility preservation (oocyte vitrification) between 2013 and 2016. The women were divided into three groups: breast cancer, lymphoma or other cancer. All of them had been recently diagnosed with cancer, with gonadotoxic treatment scheduled, and had oocyte vitrification after ovarian stimulation with antagonist protocol.

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Objective(s): Beta human chorionic gonadotrophin (β-hCG) is secreted by placenta and detectable in maternal serum a few days after embryo implantation. The evolution of β-hCG serum levels is commonly used to confirm early pregnancy and differentiate normal pregnancies from others, either in spontaneous pregnancies or after IVF. However, little is known on the eventual link between blastocyst quality in IVF, pregnancy outcome, and βhCG kinetics.

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Embryo morphology assessment performs relatively poorly in predicting implantation. Embryo aneuploidy screening (PGS) has recently improved, but its clinical value is still debated, and the development of a cheap non-invasive method for the assessment of embryo ploidy status is a highly desirable goal. The growing implementation of time-lapse devices led some teams to test the effectiveness of morphokinetic parameters as predictors of embryo ploidy, with conflicting results.

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Induced pluripotent stem cells (iPSCs) have considerably impacted human developmental biology and regenerative medicine, notably because they circumvent the use of cells of embryonic origin and offer the potential to generate patient-specific pluripotent stem cells. However, conventional reprogramming protocols produce developmentally advanced, or primed, human iPSCs (hiPSCs), restricting their use to post-implantation human development modeling. Hence, there is a need for hiPSCs resembling preimplantation naive epiblast.

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Purpose: The purpose of our study was to use time-lapse in order to evaluate the impact of sperm origin (fresh ejaculate or surgically retrieved) on embryo morphokinetic parameters and clinical outcome in intracytoplasmic sperm injection (ICSI) cycles.

Methods: This retrospective monocentric study was conducted in 485 unselected couples undergoing 604 ICSI cycles with embryo culture in the Embryoscope®. Among them, 445 couples underwent ICSI cycle with fresh ejaculated sperm and 40 with surgically retrieved sperm (26 with testicular sperm and 14 with epididymal sperm).

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