Scalable long read self-correction and assembly polishing with multiple sequence alignment.

Third-generation sequencing technologies allow to sequence long reads of tens of kbp, that are expected to solve various problems. However, they display high error rates, currently capped around 10%. Self-correction is thus regularly used in long reads analysis projects.

ELECTOR: evaluator for long reads correction methods.

The error rates of third-generation sequencing data have been capped >5%, mainly containing insertions and deletions. Thereby, an increasing number of diverse long reads correction methods have been proposed. The quality of the correction has huge impacts on downstream processes.

Strategies for the Identification of Bioactive Neuropeptides in Vertebrates.

Neuropeptides exert essential functions in animal physiology by controlling , reproduction, development, growth, energy homeostasis, cardiovascular activity and stress response. Thus, identification of neuropeptides has been a very active field of research over the last decades. This review article presents the various methods used to discover novel bioactive peptides in vertebrates.

A time of change: dietary reconstruction of the Merovingian cemetery of Norroy-le-Veneur, France.

The aim of this work was to analyse the diet of a Merovingian population sample of 80 individuals buried at Norroy-le-Veneur, France, with regard to their social status and chronology. A carbon and nitrogen stable isotope analysis of human adult bone collagen and related fauna from the same cemetery showed a diet based primarily on C plants, supplemented with animal protein in a range comparable to other contemporary sites. No significant contribution of C plants (e.

Hybrid correction of highly noisy long reads using a variable-order de Bruijn graph.

Motivation: The recent rise of long read sequencing technologies such as Pacific Biosciences and Oxford Nanopore allows to solve assembly problems for larger and more complex genomes than what allowed short reads technologies. However, these long reads are very noisy, reaching an error rate of around 10-15% for Pacific Biosciences, and up to 30% for Oxford Nanopore. The error correction problem has been tackled by either self-correcting the long reads, or using complementary short reads in a hybrid approach.

Erratum: The Beaker phenomenon and the genomic transformation of northwest Europe.

This corrects the article DOI: 10.1038/nature25738.

The Beaker phenomenon and the genomic transformation of northwest Europe.

From around 2750 to 2500 bc, Bell Beaker pottery became widespread across western and central Europe, before it disappeared between 2200 and 1800 bc. The forces that propelled its expansion are a matter of long-standing debate, and there is support for both cultural diffusion and migration having a role in this process. Here we present genome-wide data from 400 Neolithic, Copper Age and Bronze Age Europeans, including 226 individuals associated with Beaker-complex artefacts.

EVA: Exome Variation Analyzer, an efficient and versatile tool for filtering strategies in medical genomics.

Background: Whole exome sequencing (WES) has become the strategy of choice to identify a coding allelic variant for a rare human monogenic disorder. This approach is a revolution in medical genetics history, impacting both fundamental research, and diagnostic methods leading to personalized medicine. A plethora of efficient algorithms has been developed to ensure the variant discovery.