Using low-shear aerated and agitated bioreactor for producing two specific laccases by trametes versicolor cultures induced by 2,5-xylidine: Process development and economic analysis.

Laccase isoforms from basidiomycetes exhibit a superior redox potential compared to commercially available laccases obtained from ascomycete fungi, rendering them more reactive toward mono-substituted phenols and polyphenolic compounds. However, basidiomycetes present limitations for large-scale culture in liquid media, restraining the current availability of laccases from this fungal class. To advance laccase production from basidiomycetes, a newly designed 14-L low-shear aerated and agitated bioreactor provided enzyme titers up to 23.

Adjustment of the Operational Parameters of an Unconventional Integrated and Illuminated Internally Photobioreactor (ILI-PBR) for the Batch Autotrophic Cultivation of the Chlorella minutissima, Using the Taguchi Method.

Microalgae crops targeting biotechnological applications are conducted in photobioreactors, which allow the adjustment and control of parameters of luminosity, agitation and mixing in the cultivation medium to promote better cell growth and accumulation of metabolites. In this sense, the present work used the Taguchi method to find the best adjustment of the operational parameters of an unconventional photobioreactor denominated internally illuminated integrated photobioreactor (ILI-PBR) with LED light aiming at elevating the biomass concentration, volumetric biomass productivity, and volumetric lipid productivity of Chlorella minutissima microalgae cultivated under autotrophic regime. The effects of the factors were evaluated: illumination (blue, white and red); photoperiod (12 h light:12 h dark, 1 h light:1 h dark, and 24 h clear:0 h dark); aeration (0, 3 vvm, 0.

Oxygen supply in Bacillus thuringiensis fermentations: bringing new insights on their impact on sporulation and δ-endotoxin production.

The growth kinetics, sporulation, and toxicity of Bacillus thuringiensis var. israelensis were evaluated through the analysis of batch cultures with different dissolved oxygen (DO) profiles. Firstly, DO was maintained constant at 5%, 20%, or 50% throughout fermentation in order to identify the most suitable one to improve the main process parameters.