Characterizing the spatial distributions of soil biota at a legacy base metal mine using environmental DNA.

Characterizing the distribution of biota in response to contaminants is a critical element of site risk assessments. In this study we investigated the spatial distributions of biota and soil chemistry data in surface soil from Sunny Corner, a legacy base metal sulfide mine, Australia. Our results showed that copper (Cu), zinc (Zn), arsenic (As) and lead (Pb) in the surface soil exceeded Australian national soil quality guidelines and posed risks to the environment.

Impact assessment of ephemeral discharge of contamination downstream of two legacy base metal mines using environmental DNA.

Mining and processing metalliferous ores can degrade the environment well beyond the footprint of the mine, particularly where on-site containment and post-mining remediation has been insufficient to prevent releases of solid and aqueous mine wastes. In this study, we investigated the potential of sediment and water chemistry coupled with environmental (e)DNA metabarcoding to evaluate discrete and cumulative ecological impacts of two legacy base metal (copper (Cu), zinc (Zn), lead (Pb)) mines (Peelwood and Cordillera) which discharge metals via ephemeral tributaries into perennial Peelwood Creek. Although the two mine streams exceeded Australian guidelines for sediment and freshwater quality for Cu, Zn and Pb, Peelwood Creek had relatively low sediment and water metal concentrations, suggesting a low potential for environmental toxicity.

Application of environmental DNA for assessment of contamination downstream of a legacy base metal mine.

Acid Rock Drainage (ARD) from legacy mines can negatively impact the biota in sediments and waters for tens of kilometers downstream. Here we used environmental (e)DNA metabarcoding to assess the impacts of metal contaminants on biota in sediment and water downstream of a legacy base metal sulfide mine in southeastern Australia, as exemplar of similar mines elsewhere. Concentrations of metals in water were below Australian water quality guideline values at 20 km downstream for copper (Cu), 40 km downstream for zinc (Zn) and 10 km downstream for lead (Pb).

History of environmental contamination at Sunny Corner Ag-Pb-Zn mine, eastern Australia: A meta-analysis approach.

Environmental impacts associated with mining can be important even after cessation of ore extraction, particularly where sites are abandoned and unremediated. Acid Mine Drainage (AMD) is a common concern in such legacy mines where sulfide ores were extracted. AMD can introduce large concentrations of heavy metals to aquatic systems and contaminate the environment for many kilometres downstream of old mines.

Assessment of legacy mine metal contamination using ants as indicators of contamination.

Most legacy mines contributed to contamination of the environment before and after cessation of mining. Contamination from waste rock, slag and tailings can introduce large concentrations of metals and metalloids to the surface soil and downstream sediments. Since ants are able to accumulate metals in their bodies, we investigated the possibility of using the elemental compositions of ants as indicators of metals at legacy mines developed on ores rich in copper (Cu), zinc (Zn), arsenic (As), silver (Ag) and lead (Pb).

Effects of red earthworms (Eisenia fetida) on leachability of lead minerals in soil.

Contamination of soils by metals and metalloids is an important environmental problem in many residential and industrial sites around the world. Lead is a common contaminant, which enters the soil through mining, industrial activities and waste disposal. A range of technologies can be used to remediate soil lead, however most remediation technologies adversely affect the environment and particularly soil biota.