Publications by authors named "Armin Holle"
We have investigated the use of a Gaussian beam laser for MALDI Imaging Mass Spectrometry to provide a precisely defined laser spot of 5 μm diameter on target using a commercial MALDI TOF instrument originally designed to produce a 20 μm diameter laser beam spot at its smallest setting. A Gaussian beam laser was installed in the instrument in combination with an aspheric focusing lens. This ion source produced sharp ion images at 5 μm spatial resolution with signals of high intensity as shown for images from thin tissue sections of mouse brain.
View Article and Find Full Text PDFMatrix assisted laser desorption/ionization (MALDI) applications, such as proteomics, genomics, clinical profiling and MALDI imaging, have created a growing demand for faster instrumentation. Since the commonly used nitrogen lasers have throughput and life span limitations, diode-pumped solid-state lasers are an alternative. Unfortunately this type of laser shows clear performance limitations in MALDI in terms of sensitivity, resolution and ease of use, for applications such as thin-layer sample preparations, acceptance of various matrices (e.
View Article and Find Full Text PDFArticle Synopsis
- A new type of mass spectrometer called MALDI LIFT-TOF/TOF MS is introduced, which helps scientists analyze tiny amounts of proteins very accurately.
- This machine can break down proteins in three different ways to study their parts and works automatically, making the process much easier for researchers.
- With this advanced tool, scientists can identify proteins better and even find mistakes or changes in protein sequences, helping improve our understanding of how proteins work in living things.