Genotoxicity and cytotoxicity of sevoflurane in two human cell lines in vitro with ionizing radiation.

Objective: To determine the in vitro toxicity of different concentrations of sevoflurane in cells exposed to X-ray.

Methods: The genotoxic effects of sevofluorane were studied by means of the micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes. Subsequently, its cytotoxic effects on PNT2 (normal prostate) cells was determined using the cell viability test (MTT) and compared with those induced by different doses of X-rays.

Zoledronic acid and radiation: toxicity, synergy or radiosensitization?

Introduction: Zoledronic acid (Z) is a bisphosphonate used in hypercalcaemia-related cancer, in complications for bone metastasis and in postmenopausal osteoporosis and it has been related to osteoradionecrosis, especially when associated with radiation to the head and neck structures.

Objectives: To determine the radiosensitization capacity of zoledronic acid in the combined treatment with ionizing radiation (IR) by evaluating its genotoxic and cytotoxic capacities in non-tumoral cells.

Materials And Methods: The genotoxic effect of Z was studied by means of the micronucleus test in cytokinesis-blocked cells of human lymphocytes irradiated before and after a 2 Gy irradiation, while the cytotoxic effect was studied by a cell viability test in the PNT2 cell line before and after exposure to different X-ray doses (0-20 Gy) in four groups (Z alone, radiation alone, Z + IR and IR + Z).

Chemical genoprotection: reducing biological damage to as low as reasonably achievable levels.

Objectives: The aim of this study was to evaluate the antioxidant substances present in the human diet with an antimutagenic protective capacity against genotoxic damage induced by exposure to X-rays in an attempt to reduce biological damage to as low a level as reasonably possible.

Methods: Ten compounds were assessed using the lymphocyte cytokinesis-block micronucleus (MN) cytome test. The compounds studied were added to human blood at 25 μM 5 min before exposure to irradiation by 2 Gy of X-rays.

The status of Spain's dental practice following the European Union directive concerning radiological installations: 11 years on (1996-2007).

Objectives: The aim of this study was to assess the influence of European Union legislation on dental radiology practice in Spain and the reduction in doses administered in dental radiological installations 11 years after its introduction.

Methods: A total of 19 079 official reports on dental surgeries from 16 Spanish autonomous regions published between 1996 and 2007 were studied. We analysed the physical characteristics of the X-ray units, anomalies, film processing, exposure times and mean radiation doses administered in clinical situations.

Collimator with filtration compensator: clinical adaptation to meet European Union recommendation 4F on radiological protection for dental radiography.

Objective: Our aim was to develop a compensated filtration collimator for use in paediatric patients undergoing cephalometric radiography that reduces the radiation dose administered and fulfils recommendation 4F of the European guidelines on radiation protection in dental radiology.

Methods: An easy to use filtration-compensated collimator was constructed of plastic, lead and aluminium and used randomly with a group of 32 children (mean age 11 years) undergoing cephalometric radiography before receiving orthodontic treatment. The radiation doses administered to patients (eye lens and thyroid, submandibular and parotid glands) and to the chassis of the radiographic equipment were determined.

Liposoluble antioxidants provide an effective radioprotective barrier.

Ionising radiation causes the massive generation of reactive oxygen species and induces cellular DNA damage. The antioxidant, protective effects of several compounds against gamma-ray-induced chromosomal damage were determined by the micronucleus test, evaluating the reduction in the frequency of micronuclei in cytokinesis-blocked human lymphocytes. The compounds studied were added to human blood at 25 microM, 5 min before or after irradiation with 2 Gy of caesium-137.

[Complications at the first month following various types of intracoronary stents, implanted with high pressure without intracoronary ultrasonography or anticoagulation].

Background: Coronary stents have proved their efficacy in bail-out situations and restenosis. Nevertheless, the high incidence of subacute thrombosis and vascular and bleeding complications limits its use.

Objectives: To evaluate the clinical complications during the first month of three different types of stents, implanted with high pressure, without ultrasound guidance or anticoagulation.