Publications by authors named "Armando A Losada"
Background: The biosynthesis pathway of benzoxazole compounds caboxamycin and nataxazole have been recently elucidated. Both compounds share one of their precursors, 3-hydroxyanthranilate (two units in the case of nataxazole). In addition, caboxamycin structure includes a salicylate moiety while 6-methylsalycilate is the third scaffold in nataxazole.
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- Streptomyces sp. NTK937 produces the antibiotic caboxamycin and its methyl ester derivative, O-methylcaboxamycin, with the latter's synthesis involving genes located outside the primary biosynthetic cluster.
- The caboxamycin gene cluster includes one regulatory gene and nine structural genes, of which five are essential for its production, while the other five have paralogues that can compensate if their cluster counterparts are inactive.
- Genetic manipulation led to the discovery of a new compound, 3'-hydroxycaboxamycin, formed from the interaction between the caboxamycin and enterobactin biosynthetic pathways in a mutant strain lacking salicylate synthase.
Streptomyces sp. Tü 6176, producer of cytotoxic benzoxazoles AJI9561, nataxazole, and 5-hydroxy-nataxazole, has been found to produce a fourth benzoxazole, UK-1. All derive from 3-hydroxy-anthranilate synthesized by the nataxazole biosynthesis machinery.
View Article and Find Full Text PDFStreptomyces sp. strain NTK 937 is the producer of the benzoxazole antibiotic caboxamycin, which has been shown to exert inhibitory activity against Gram-positive bacteria, cytotoxic activity against several human tumor cell lines, and inhibition of the enzyme phosphodiesterase. In this genome announcement, we present a draft genome sequence of Streptomyces sp.
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