Effect of Plant Preservative Mixture on Endophytic Bacteria Eradication from In Vitro-Grown Apple Shoots.

Endophytic contaminants are a common problem for the in vitro propagation of woody plants and have significant economic repercussions for the conservation of plant genetic resources and commercial micropropagation. In this study, first, the microbial contamination that appeared around the base of in vitro-grown apple shoots was identified as . Then, plant preservative mixture (PPM) was used as a bactericidal agent in plant tissue culture.

Multiple Plant Regeneration from Embryogenic Calli of (Thunb.) Steud.

The aim of this paper was to study the effect of plant growth regulators on callus induction and morphogenesis using various explants of to develop an efficient plant regeneration protocol. Different plant organ sections (leaves, apical shoot tips, petals, nodes, and internodes) were cultured as explants to identify the best explants responsive to callus induction and plant regeneration. Explants were cultivated on MS media supplemented with different concentrations of plant growth regulators (TDZ (Thidiazuron), BAP (6-Benzylaminopurine), kinetin, and NAA (1-Naphthaleneacetic acid).