Publications by authors named "Arman Karshenas"
Despite the sequencing revolution, large swaths of the genomes sequenced to date lack any information about the arrangement of transcription factor binding sites on regulatory DNA. Massively Parallel Reporter Assays (MPRAs) have the potential to dramatically accelerate our genomic annotations by making it possible to measure the gene expression levels driven by thousands of mutational variants of a regulatory region. However, the interpretation of such data often assumes that each base pair in a regulatory sequence contributes independently to gene expression.
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- Understanding the role of transcription factor binding sites in gene regulation is a critical and complex issue in biology, particularly for multicellular organisms.
- Massively parallel reporter assays (MPRAs) enable high-throughput measurement of gene expression from numerous regulatory sequences in cell cultures but face limitations in whole organisms due to the need for DNA library integration.
- Researchers developed a system using base editing technology to introduce targeted mutations in the genomes of cell cultures and whole organisms, significantly improving the ability to study enhancer functions by using multiple guide RNAs for efficient mutagenesis.
Introduction: Many countries with an early outbreak of SARS-CoV-2 struggled to gauge the size and start date of the epidemic mainly due to limited testing capacities and a large proportion of undetected asymptomatic and mild infections. Iran was among the first countries with a major outbreak outside China.
Methods: We constructed a globally representative sample of 802 genomes, including 46 samples from patients inside or with a travel history to Iran.