Methods for Testing Repellents Against Bed Bugs (Hemiptera: Cimicidae).

Bed bug repellents should not only prevent humans from being bitten but impede an infestation of personal belongings. Only a few test proposals for evaluating the efficacy of repellents against bed bugs have been published so far. In the present study, two test systems were assessed for efficacy testing with five potential bed bug repellents (cinnamon oil, icaridin, N,N-diethyl-3-methylbenzamide (DEET), permethrin, and margosa extract).

Detection of target-site and metabolic resistance to pyrethroids in the bed bug Cimex lectularius in Berlin, Germany.

Knockdown-resistance (kdr) against pyrethroids in bed bugs (Cimex lectularis) is associated with the presence of several point mutations in the voltage-sensitive sodium channel α-subunit gene and/or an increased metabolic detoxification by cytochrome P450 monooxygenases (CYPs). In the present study, pyrosequencing assays were developed to quantify the presence of the kdr substitutions (V419L or L925I substitution) in bed bugs in Berlin, Germany. In 14 of 17 bed bug field strains, pyrosequencing revealed the presence of the substitution L925I with allele frequencies between 30% and 100%.

A Novel Simulated-Use Test for Determining the Efficacy of Insecticides Against Bed Bugs (Hemiptera: Cimicidae).

The common bed bug Cimex lectularius L. has undergone a worldwide expansion in recent years, due to increased opportunities for dispersal and development of insecticide resistance. For successful control, efficacy testing of products against bed bugs and determination of insecticide resistance under practical conditions are of outstanding importance.