Exploring the coordination chemistry of ruthenium complexes with lysozymes: structural and in-solution studies.

This study presents a comprehensive structural analysis of the adducts formed upon the reaction of two Ru(III) complexes [HIsq][-RuCl(dmso)(Isq)] () and [HInd][-RuCl(dmso)(HInd)] () (where HInd-indazole, Isq-isoquinoline, analogs of NAMI-A) and two Ru(II) complexes, -[RuCl(dmso)] () and -[RuCl(dmso)] (), with hen-egg white lysozyme (HEWL). Additionally, the crystal structure of an adduct of human lysozyme (HL) with ruthenium complex, [HInd][-RuCl(dmso)(HInd)] was solved. X-ray crystallographic data analysis revealed that all studied Ru complexes, regardless of coordination surroundings and metal center charge, coordinate to the same amino acids (His15, Arg14, and Asp101) of HEWL, losing most of their original ligands.