Analysis of the Choristoneura fumiferana nucleopolyhedrovirus genome.

The double-stranded DNA genome of Choristoneura fumiferana nucleopolyhedrovirus (CfMNPV) was sequenced and analysed in the context of other group I nucleopolyhedroviruses (NPVs). The genome consists of 129,593 bp with a G + C content of 50.1 mol%.

Identification and expression of a mouse muscle-specific CTL1 gene.

In this study, a mouse gene and cDNA encoding for a novel skeletal muscle-specific choline transporter-like protein 1 (mCTL1) were identified and mCTL1 mRNA and protein expression characterized. The mCTL1 cDNA is 2888-bp long; consisting of a 653-amino-acid open-reading frame, 8-11 putative transmembrane domains, three N-glycosylation sites and seven protein kinase C phosphorylation sites. The mCTL1 gene is localized to chromosome 4B2, at 182 kb in length, and encoded by 17 exons.

Cloning and comparative analysis of the bovine, porcine, and equine sex chromosome genes ZFX and ZFY.

A growing body of evidence suggests the involvement of sex chromosome genes in mammalian development. We report the cloning and characterization of the complete coding regions of the bovine Y chromosome ZFY and X chromosome ZFX genes, and partial coding regions of porcine and equine ZFX and ZFY genes. Bovine ZFY and ZFX are highly similar to each other and to ZFX and ZFY from other species.

Genomic organization and differential splicing of the mouse and human Pcyt2 genes.

CTP: ethanolaminephosphate cytidylyltransferase (Pcyt2) is an important regulatory enzyme in phosphatidylethanolamine and plasmalogen biosynthesis. We cloned the mouse gene mPcyt2 and established its relationship with the human homolog PCYT2. The two genes share similar size and contain two conserved catalytic domains but exhibit different exon/intron organization.