Evaluation of physiological and biochemical responses in different seasons in Surti buffaloes.

Aim: This study was conducted to evaluate the impact of hot dry, hot humid and comfortable season on physiological, hematological, biochemical, and oxidative stress parameters in Surti buffaloes.

Materials And Methods: Ten lactating Surti buffaloes of similar physiological status were selected. Based on the temperature-humidity index (THI), their natural exposure to the environment was categorized as hot dry (THI1), hot humid (THI2) and moderate winter/comfort season (THI3).

Effects of β-mercaptoethanol on in vitro maturation and glutathione level of buffalo oocytes.

Aim: The present study was carried out to evaluate the effect of supplementation of β-mercaptoethanol (β-ME) on in vitro maturation rate and glutathione (GSH) level of buffalo oocytes.

Materials And Methods: Oocytes were recovered from buffalo's ovaries collected from government approved slaughter house (near Kamela darwaza, Surat) of Surat Municipal Corporation. The obtained oocytes were in vitro matured in maturation media supplemented with 0 μM (117 oocytes), 100 μM (46 oocytes) and 200 μM (42 oocytes) concentration of β-ME.