Analysis of the gas phase of cigarette smoke by gas chromatography coupled with UV-diode array detection.

A gas chromatography method, coupled with diode array photometric spectral detection in the ultraviolet region (167-330 nm), was developed for the analysis of the gas phase of cigarette smoke. The method enabled us to identify more than 20 volatiles present in the vapor phase of cigarette smoke. In that way, all major volatile organic compounds (including aldehydes, conjugated dienes, ketones, sulfides, furans, and single-ring aromatics), as well as nitric oxide (NO) and hydrogen sulfide (H(2)S), can be analyzed in a straightforward manner through a single chromatographic run of <50-min duration.

Study of the mechanisms of cigarette smoke gas phase cytotoxicity.

Background: The cytotoxicity of cigarette smoke (CS) in humans is well-documented, but the mechanism behind CS toxicity and carcinogenicity remains unknown. We are interested in the toxicological effects of CS gas phase and the biological mechanisms of its action.

Materials And Methods: Gas phase CS cytotoxicity was measured by Wst-1 and LDH assays, in cultured cells.

The cytotoxic effect of volatile organic compounds of the gas phase of cigarette smoke on lung epithelial cells.

Health effects of cigarette smoke (CS) in humans are well known from both clinical and epidemiological studies. However, the mechanism behind CS toxicity and carcinogenicity remains mainly unknown. Recent studies have pointed to the major importance of the gas phase of CS in generating its cytotoxic effects.