Characterization of a Novel Functional Trimeric Catechol 1,2-Dioxygenase From a Isolated From the Gulf of Mexico.

Catechol 1,2 dioxygenases (C12DOs) have been studied for its ability to cleavage the benzene ring of catechol, the main intermediate in the degradation of aromatic compounds derived from aerobic degradation of hydrocarbons. Here we report the genome sequence of the marine bacterium GOM2, isolated from the southwestern Gulf of Mexico, and the biochemical characterization of its C12DO (PsC12DO). The gene, encoding PsC12DO of 312 amino acid residues, was cloned and expressed in Many C12DOs have been described as dimeric enzymes including those present in species.