Correction: Live cell single molecule tracking and localization microscopy of bioorthogonally labeled plasma membrane proteins.

Correction for 'Live cell single molecule tracking and localization microscopy of bioorthogonally labeled plasma membrane proteins' by Andres I. König et al., Nanoscale, 2020, 12, 3236-3248, DOI: 10.

Live cell single molecule tracking and localization microscopy of bioorthogonally labeled plasma membrane proteins.

Tracking the localization and mobility of individual proteins in live cells is key for understanding how they mediate their function. Such information can be obtained from single molecule imaging techniques including as Single Particle Tracking (SPT) and Single Molecule Localization Microscopy (SMLM). Genetic code expansion (GCE) combined with bioorthogonal chemistry offers an elegant approach for direct labeling of proteins with fluorescent dyes, holding great potential for improving protein labeling in single molecule applications.

A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag.

Background: In the high-resolution microscopy era, genetic code expansion (GCE)-based bioorthogonal labeling offers an elegant way for direct labeling of proteins in live cells with fluorescent dyes. This labeling approach is currently not broadly used in live-cell applications, partly because it needs to be adjusted to the specific protein under study.

Results: We present a generic, 14-residue long, N-terminal tag for GCE-based labeling of proteins in live mammalian cells.

[The real and ideal in the evaluation of functional competence among demented patients].

The progressive nature of the dementia of Alzheimer's disease has significant clinical, functional, legal and nursing implications. Traditionally, the grading of severity of the disease is based on evaluation of the patients' independence and competence. However, the term competence has been used in different respects, leading to obscuration and confusion.