T cell receptor (TCR) signaling regulates important developmental transitions, partly through induction of the E protein antagonist, Id3. Although normal γδ T cell development depends on Id3, Id3 deficiency produces different phenotypes in distinct γδ T cell subsets. Here, we show that Id3 deficiency impairs development of the Vγ3 subset, while markedly enhancing development of NKγδT cells expressing the invariant Vγ1Vδ6.
View Article and Find Full Text PDFV(D)J recombination of antigen receptor loci is a highly developmentally regulated process. During T lymphocyte development, recombination of the gene occurs in CD4CD8 double positive (DP) thymocytes and requires the enhancer (Eα). E proteins are known regulators of DP thymocyte development and have three identified binding sites in Eα.
View Article and Find Full Text PDFThe Tcra repertoire is generated by multiple rounds of Vα-Jα rearrangement. However, Tcrd recombination precedes Tcra recombination within the complex Tcra-Tcrd locus. Here, by ablating Tcrd recombination, we report that Tcrd rearrangement broadens primary Vα use to diversify the Tcra repertoire in mice.
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