Detection of Prosthetic Joint Infection Based on Magnetically Assisted Surface Enhanced Raman Spectroscopy.

Accurate and rapid diagnosis of prosthetic joint infection (PJI) is vital for rational and effective therapeutic management of this condition. Several diagnostic strategies have been developed for discriminating between infected and noninfected cases. However, none of them can reliably diagnose the whole spectrum of clinical presentations of PJI.

Highly efficient silver particle layers on glass substrate synthesized by the sonochemical method for surface enhanced Raman spectroscopy purposes.

A fast method for preparing of silver particle layers on glass substrates with high application potential for using in surface enhanced Raman spectroscopy (SERS) is introduced. Silver particle layers deposited on glass cover slips were generated in one-step process by reduction of silver nitrate using several reducing agents (ethylene glycol, glycerol, maltose, lactose and glucose) under ultrasonic irradiation. This technique allows the formation of homogeneous layers of silver particles with sizes from 80nm up to several hundred nanometers depending on the nature of the used reducing agent.

Magnetically-assisted surface enhanced raman spectroscopy (MA-SERS) for label-free determination of human immunoglobulin G (IgG) in blood using Fe3O4@Ag nanocomposite.

Development of methods allowing determination of even ultralow levels of immunoglobulins in various clinical samples including whole human blood and plasma is a particular scientific challenge, especially due to many essential discoveries in the fields of immunology and medicine in the past few decades. The determination of IgG is usually performed using an enzymatic approach, followed by colorimetric or fluorimetric detection. However, limitations of these methods relate to their complicated setup and stringent requirements concerning the sample purity.