Non-destructive quantitative analysis of pharmaceutical ointment by transmission Raman spectroscopy.

Transmission Raman spectroscopy was used to develop a non-destructive quantitative analytical model for the assay of a crystal dispersion-type ointment containing acyclovir as a model drug with a concentration of 3% w/w. The obtained Raman spectra were pre-processed by applying multiplicative scatter correction, standard normal variate, and first or second derivative by the Savitzky-Golay method to optimize the partial least squares (PLS) regression model. The optimized PLS model showed good prediction performance for 85%, 100%, and 115% label claims, with average recovery values of 100.