Publications by authors named "Argiris A Talamagas"
To understand matrix metalloproteinase-9 (MMP-9) involvement in Alzheimer's disease, we examined mechanisms mediating increased expression of MMP-9 in the presence of Abeta(1-40) and the role of MMP-9 on amyloid precursor protein (APP) processing. Up-regulation of MMP-9 expressed by SK-N-SH cells in the presence of Abeta(1-40) was mediated by alpha(3)beta(1) and alpha(2)beta(1) integrin receptors. Overexpression of MMP-9 or treatment of HEK/APP695 cells with activated recombinant MMP-9 resulted in enhanced secretion of soluble APP (sAPPalpha), a product of alpha-secretase cleavage, and reduction of Abeta release.
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- GLUTs are membrane proteins that help transport glucose, and this study focuses on their expression in kidney cells under diabetic conditions, specifically looking at GLUTs 1, 3, and 4.
- Researchers used an immortalized HGEC cell line and cultured it in different glucose concentrations to analyze GLUT expression using Western blotting and flow cytometry.
- The study found that GLUT-1 levels increased in high glucose conditions, while GLUT-4 decreased, suggesting that these changes may contribute to the early signs of diabetic kidney disease.
Integrin-mediated interactions with collagen IV and its domains were examined in a human neuroblastoma cell line (SK-N-SH). By adhesion assays we demonstrated that neuroblastoma cells bound to solid-phase intact collagen IV and synthetic cell-binding peptide HEP-III, derived from the collagenous part of the molecule, but not to the main noncollagenous NC1 domain or to the synthetic cell-binding peptide HEP-I, derived from this domain. Monoclonal antibodies against beta1, alpha3, and alpha(v)beta3 integrins resulted in inhibition of cell binding to collagenous substrates by 95, 30, and 35%, respectively.
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