Morphological, Molecular and Metabolic Characterization of the Pigmented Fungus .

Chaetomiaceae fungi are ascosporulating fungi whose importance as human pathogens has been frequently ignored. In the current study, a new isolate of the genus was described. The fungus was isolated from the soil of Wadi Om Nefa'a, Hurghada in the Red Sea Governorate, Egypt.

Isolation, Characterization, and Metabolic Profiling of from the Aquatic Plant .

The goal of the present study was to investigate the antibacterial properties, enzyme production, and metabolic profiling of a new strain isolated from the submerged aquatic plant . Furthermore, the fungus' morphological characterization and DNA sequencing have been described. The fungus has been identified and submitted to the GenBank as isolate EG19 and the fungus ID is MK387081.

Metabolic profiling, in vitro propagation, and genetic assessment of the endangered rare plant Anarrhinum pubescens.

Background: Anarrhinum pubescens Fresen. (Plantaginaceae) is a rare plant, endemic to the Saint Catherine area, of South Sinai, Egypt. Earlier studies have reported the isolation of cytotoxic and anti-cholinesterase iridoid glucosides from the aerial parts of the plant.

Comparative Analysis of Au and Au@SiO Nanoparticle-Protein Interactions for Evaluation as Platforms in Theranostic Applications.

Gold nanoparticles are utilized in a variety of sensing and detection technologies because of their unique physiochemical properties. Their tunable size, shape, and surface charge enable them to be used in an array of platforms. The purpose of this study is to conduct a thorough spectroscopic characterization of Au and functionalized hybrid Au@SiO nanoparticles under physiological conditions and in the presence of two proteins known to be abundant in serum, bovine serum albumin and human ubiquitin.

New iridoid glycosides from .

Six metabolites () were isolated from the aerial parts of Fresen. (Plantaginaceae) growing in Saint Catherine region in Egypt; two of them ( and ) are here reported to be newly identified naturally occurring iridoids. The isolated metabolites were identified as 6--foliamenthoyl-(6'--cinnamoyl)-antirrhinoside (), 6'--cinnamoyl-antirrhinoside (), the iridoid dimer, pubescensoside (), antirrhinoside (), 10-hydroxy-antirrhinoside (), and the flavonoid, diosmin ().

NMR Analysis Reveals a Wealth of Metabolites in Root-Knot Nematode Resistant Roots of Watermelon Plants.

Citrullus amarus ( CA ) (previously known as Citrullus lanatus var. citroides ) accessions collected in southern Africa are known to have resistance to root-knot nematodes (RKN) and are suitable rootstocks for grafted watermelon. The objective of this study was to conduct a comparative metabolomics analysis and identify unique metabolites in roots of CA accessions versus roots of watermelon cultivars ( Citrullus lanatus (Thunb.

Differential roles of melatonin in plant-host resistance and pathogen suppression in cucurbits.

Since the 1950s, research on the animal neurohormone, melatonin, has focused on its multiregulatory effect on patients suffering from insomnia, cancer, and Alzheimer's disease. In plants, melatonin plays major role in plant growth and development, and is inducible in response to diverse biotic and abiotic stresses. However, studies on the direct role of melatonin in disease suppression and as a signaling molecule in host-pathogen defense mechanism are lacking.

NMR-based metabolomic analysis of wild, greenhouse, and in vitro regenerated shoots of subsp. with GC-MS assessment of proximadiol.

subsp is a wild plant distributed in subtropical and east Africa extending from the north to the southern parts of Egypt. Widely used in folk medicine, it is the source of the diuretic sesquiterpene proximadiol. Nuclear magnetic resonance metabolomic analysis of polar extracts of shoots from wild, greenhouse, somatic embryos, and direct and indirect organogenic in vitro cultures was carried out.

NMR Spectroscopy Identifies Metabolites Translocated from Powdery Mildew Resistant Rootstocks to Susceptible Watermelon Scions.

Powdery mildew (PM) disease causes significant loss in watermelon. Due to the unavailability of a commercial watermelon variety that is resistant to PM, grafting susceptible cultivars on wild resistant rootstocks is being explored as a short-term management strategy to combat this disease. Nuclear magnetic resonance-based metabolic profiles of susceptible and resistant rootstocks of watermelon and their corresponding susceptible scions (Mickey Lee) were compared to screen for potential metabolites related to PM resistance using multivariate principal component analysis.

Cold adaptation shapes the robustness of metabolic networks in Drosophila melanogaster.

When ectotherms are exposed to low temperatures, they enter a cold-induced coma (chill coma) that prevents resource acquisition, mating, oviposition, and escape from predation. There is substantial variation in time taken to recover from chill coma both within and among species, and this variation is correlated with habitat temperatures such that insects from cold environments recover more quickly. This suggests an adaptive response, but the mechanisms underlying variation in recovery times are unknown, making it difficult to decisively test adaptive hypotheses.

NMR-based metabolomics study of the biochemical relationship between sugarcane callus tissues and their respective nutrient culture media.

The culture of sugarcane leaf explant onto culture induction medium triggers the stimulation of cell metabolism into both embryogenic and non-embryogenic callus tissues. Previous analyses demonstrated that embryogenic and non-embryogenic callus tissues have distinct metabolic profiles. This study is the follow-up to understand the biochemical relationship between the nutrient media and callus tissues using one-dimensional (1D (1)H) and two-dimensional (2D (1)H-(13)C) NMR spectroscopy followed by principal component analysis (PCA).

Tissue-Specific Metabolic Profile Study of Using Nuclear Magnetic Resonance Spectroscopy.

, an important multipurpose crop, is rich in various phytochemicals: flavonoids, antioxidants, vitamins, minerals and carotenes. The purpose of this study was to profile the groups of metabolites in leaf and stem tissues of . Various sugars, amino acids, and organic acid derivatives were found in all of the tissues with different profiles/peak intensities depending on the tissue.

Identification of isethionic acid and other small molecule metabolites of Fragilariopsis cylindrus with nuclear magnetic resonance.

Nuclear magnetic resonance (NMR) spectroscopy has been used to obtain metabolic profiles of the polar diatom Fragilariopsis cylindrus, leading to the identification of a novel metabolite in this organism. Initial results from an ongoing metabolomics study have led to the discovery of isethionic acid (2-hydroxyethanesulfonic acid, CAS: 107-36-8) as a major metabolite in F. cylindrus.

NMR-derived folate-bound structure of dihydrofolate reductase 1 from the halophile Haloferax volcanii.

Folate binds to dihydrofolate reductase (DHFR) to form a binary complex whose structure maintains the overall configuration of the enzyme; however, some significant changes are evident when a comparison is made to the enzyme. The structure of DHFR1 from the halophilic Halopherax volcanii was solved in its folate-bound form using nuclear magnetic resonance spectroscopy. NOE data obtained from the (15)N-NOESY-HSQC and (13)C-NOESY-HSQC experiments of the triply labeled ((1)H, (13)C, and (15)N) binary complex were used as input for the structure calculation with the Crystallography and Nuclear Magnetic Resonance System program.

Structure in an extreme environment: NMR at high salt.

Proteins from halophiles have adapted to challenging environmental conditions and require salt for their structure and function. How halophilic proteins adapted to a hypersaline environment is still an intriguing question. It is important to mimic the physiological conditions of the archae extreme halophiles when characterizing their enzymes, including structural characterization.

NMR assignments of the binary hvDHFR1:folate complex.

A better understanding of how salt affects enzyme activity can be gained via NMR studies of binary hvDHFR1:folate complex. Chemical shift assignments of the 17.9 kDa enzyme with bound substrate prepare the way for ongoing research of the effects of salt on enzyme flexibility through relaxation studies.