Partial Purification and Characterization of the Recombinant Benzaldehyde Dehydrogenase from UKMP-5M.

Background: Benzaldehyde dehydrogenase (BZDH) is encoded by the that catalyzes the conversion of benzaldehyde into benzoate in many pathways such as toluene degradation.

Objectives: In this study, the gene from UKMP-5M was expressed in , purified, and characterized.

Materials And Methods: The was amplified and cloned in .

Expression, purification and kinetic characterization of recombinant benzoate dioxygenase from UKMP-5M.

In this study, benzoate dioxygenase from UKMP-5M was catalyzed by oxidating the benzene ring to catechol and other derivatives. The benzoate dioxygenase (A gene) from UKMP-5M was then expressed, purified, characterized, The A gene was amplified (642 bp), and the product was cloned into a pGEM-T vector. The recombinant plasmid pGEMT-benA was digested by double restriction enzymes I and III to construct plasmid pET28b-benA and was then ligated into BL21 (DE3).