Comparison of the Colony Reduction Effect of Two Wavelengths of Diode Lasers With Three Methods of Root Canal Irrigation: An In Vitro Study.

The basis of successful root canal therapy is the reduction of microorganisms. The aim of this in vitro study was to compare the antibacterial effect of three different irrigation methods with two laser wavelengths on biofilm. Ninety-five single-canal teeth were prepared, sterilized, and divided randomly into a negative control, a positive control, and five test groups.

Comparative study of the pure AH Plus sealer and its combination with triple antibiotic paste at different concentrations on bacteria.

Background: In this study, the effects of pure AH Plus sealer and its combination with triple antibiotic paste at different concentrations on bacteria have been investigated.

Materials And Methods: This study was accomplished by the means of a triple antibiotic paste combination (minocycline, metronidazole, and ciprofloxacin) at different concentrations (0%, 1%, 5%, 10%, and 25%) with AH Plus sealer on bacteria. Sealers were set in an incubator for 1 h, 1 day, 3 days, and 7 days, and then 10 µL of bacteria solution was placed on all samples except the negative control group.

Assessment of Antimicrobial Activity of Chitosan, ZnO, and Urtica dioica-ZnO NPs Against Staphylococcus aureus Isolated from Diabetic Ulcers.

Staphylococcus aureus (S. aureus) is considered as one of the challenging ulcer infections in diabetic patients especially those who have acquired antibiotic-resistant infections. Nanotechnology products have enormous potential to treat diseases including infectious diseases.

Cyanobacterial biocrust alters soil physical properties reducing soil erosion and aerosol production.

Aerosol emission by wind erosion in the arid and semi-arid areas of the world, is of environmental and health significance. Different methods have been used to mitigate aerosol emission among which the biological methods may be the most efficient ones. Although previously investigated, more research is essential to determine how the use of exopolysaccharide (biocrust)-producing cyanobacteria may affect soil physical properties.

Chromium (VI) bioremoval from contaminated wastewater using Pseudomonas aeruginosa ATHA23 producing biofilm supported on clinoptilolite.

More has yet to be investigated on the increased efficiency of microbes for the removal of heavy metals from industrial wastewaters. The objective was to determine the Cr (VI) bioabsorption and bioreduction ability of biofilm-producing bacteria supported on clinoptilolite from contaminated aqueous solutions. Chromium (VI)-tolerant bacteria, namely Pseudomonas aeruginosa ATHA23, were identified by biochemical methods and 16S rDNA sequencing and were deposited in NCBI (accession number: KF680991).

Vermicomposting of sewage sludge with organic bulking materials to improve its properties.

Adverse properties of sewage sludge such as high concentration of toxic metals and the presence of pathogens have limited its use in agriculture. In this study, the effect of vermicomposting on some important chemical properties of sewage sludge was investigated as a factorial arrangement based on a completely randomized design with three replications. The studied factors were bulking agents in three concentrations (0, 5, and 10% v/v), earthworm (Eisenia fetida) in 2 different concentrations (0 and 50 adult earthworms/6 kg sewage sludge), and retention times in three levels (30, 60, and 90 days).

Anti-adherence and anti-bacterial activities of resin extract against strongly adherent strains.

Background: The reduction of from the oral cavity or its adherence to tooth surfaces can prevent or decrease the progression of caries. In this study, the antimicrobial and anti-adherence properties of () resin (Essential oil [EO] and methanolic extract [ME]) were investigated on strains.

Materials And Methods: In this experimental study, the growth rate, biofilm formation ability, and antibiotic susceptibility profile of ATCC35668 and 3 strains isolated from caries lesions were studied.

antimicrobial activity of mineral trioxide aggregate, Biodentine, and calcium-enriched mixture cement against , , and using the agar diffusion technique.

Background: This study assessed the antimicrobial activity of Biodentine, mineral trioxide aggregate (MTA), and calcium-enriched mixture (CEM) cement against , , and .

Materials And Methods: In this study, microbial suspensions were inoculated onto agar plates. The antimicrobial effects of MTA, Biodentine and CEM cement were assessed against , , and by the agar diffusion test.

Toxic trace element resistance genes and systems identified using the shotgun metagenomics approach in an Iranian mine soil.

This study aimed to identify the microbial communities, resistance genes, and resistance systems in an Iranian mine soil polluted with toxic trace elements (TTE). The polluted soil samples were collected from a mining area and compared against non-polluted (control) collected soils from the vicinity of the mine. The soil total DNA was extracted and sequenced, and bioinformatic analysis of the assembled metagenomes was conducted to identify soil microbial biodiversity, TTE resistance genes, and resistance systems.

The anti-tumor activity of exopolysaccharides from Pseudomonas strains against HT-29 colorectal cancer cell line.

The anti-tumor activity of extracted exopolysaccharides (EPSs) (without any side effects) of Pseudomonas aeruginosa on HT-29 colorectal cancer cell line has not been previously investigated. The extraction and partial characterization of EPS from the strains of P. aeruginosa including A (CIP A22(PTCC1310)), and B (a clinical strain) were performed.

Application of artificial neural networks to describe the combined effect of pH, time, NaCl and ethanol concentrations on the biofilm formation of Staphylococcus aureus.

Biofilms are organized communities, adherent to the surface and resistant to adverse environmental and antimicrobial agents. So, its control is very important. Staphylococcus aureus is an opportunistic pathogen with the biofilm-forming ability that causes numerous problems in the medicine and food industry.

biosurfactant inhibits biofilm formation and gene expression of caries-inducing .

Background: It is cleared that some probiotic strains inhibit biofilm formation of oral bacteria, but its mechanisms are not clearly understood yet. It is proposed that one of the mechanisms can be biosurfactant production, a structurally diverse group of surface-active compounds synthesized by microorganisms. Hence, this study focused on the evaluation of the anti-biofilm and antiadhesive activities of the derived-biosurfactant against and its effect on and genes expression level.

Effect of Probiotic Yogurt and Xylitol-Containing Chewing Gums on Salivary S Mutans Count.

Background And Aims: In addition to improving gastrointestinal health and intestinal microflora, probiotic bacteria have been recently suggested to decrease cariogenic agents in the oral cavity. The aim of this study was to investigate the effects of probiotic yogurt and xylitol-containing chewing gums on reducing salivary Streptococcus mutans levels.

Study Design: This randomized clinical trial recruited 50 female students with over 10 colony forming units S.

Tannic acid degradation by Klebsiella strains isolated from goat feces.

Background And Objectives: Tannins are toxic polyphenols that either bind and precipitate or condense proteins. The high tannin content of some plants is the preliminary limitation of using them as a ruminant feed. So, the aim of this study was the isolation and characterization of tannic acid degrading bacterial strains from goat feces before and after feeding on Pistachio-Soft Hulls as tannin rich diet (TRD).

Effects of biosurfactant produced by Lactobacillus casei on gtfB, gtfC, and ftf gene expression level in S. mutans by real-time RT-PCR.

Background: The Streptococci are the pioneer strains in plaque formation and Streptococcus mutans are the main etiological agent of dental plaque and caries. In general, biofilm formation is a step-wise process, which begins by adhesion of planktonic cells to the surfaces. Evidences show that expression of glucosyltransferase B and C (gtfB and gtfC) and fructosyltransferase (ftf) genes play critical role in initial adhesion of S.

Optimization of factors affecting hexavalent chromium removal from simulated electroplating wastewater by synthesized magnetite nanoparticles.

Hexavalent chromium is a mutagen and carcinogen that is of significant concern in water and wastewater. In the present study, magnetite nanoparticles (n-Mag) were investigated as a potential remediation technology for the decontamination of Cr (VI)-contaminated wastewater. Synthesized n-Mag was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and BET-N2 technology.

Effects of Lactobacillus reuteri-derived biosurfactant on the gene expression profile of essential adhesion genes (gtfB, gtfC and ftf) of Streptococcus mutans.

Background: Streptococci are the main causative agents in plaque formation and mutans streptococci are the principle etiological agent of dental plaque and caries. The process of biofilm formation is a step-wise process, starting with adhesion of planktonic cells to the surfaces. It is now a well known fact that expression of glucosyltransferases (gtfs) and fructosyltransferase (ftf) genes play a critical role in the initial adhesion of Streptococcus mutans to the tooth surface, which results in the formation of dental plaques and consequently caries and other periodontal diseases.

Typing of Streptococcus mutans strains isolated from caries free and susceptible subjects by multilocus enzyme electrophoresis.

This study was evaluated the clonal diversity of Streptococcus mutans in caries-free and caries-active subjects using MLEE. Strains from caries-free subjects were grouped in a single taxon. Unrooted dendrogram showed that different strains clustered in four different clades, also showed that more than one clonal type can be found in a same individual.

The study of Nickel Resistant Bacteria (NiRB) isolated from wastewaters polluted with different industrial sources.

Background: Pollution due to the heavy metals is a problem that may have negative consequences on the hydrosphere. One of the best procedures in removing the toxic metals from the environment is using metal resistant bacteria.

Results: In the present study eight nickel resistant bacteria were isolated from industrial wastewaters.

The Comparison of Proteins Elaborated by Streptococcus mutans Strains Isolated from Caries Free and Susceptible Subjects.

Objective(s): The oral streptococci especially mutans Streptococci are related with the development of caries in humans. However, they are commonly distributed not only in populations with moderate or high caries incidence but also in populations having no or low caries experience. So, in this study, the differences between protein profiles of Streptococcus mutans from different sources were compared.

The effect of a probiotic strain (Lactobacillus acidophilus) on the plaque formation of oral Streptococci.

The objective of this study was to investigate the ability of biofilm formation among mutans and non mutans oral streptococci and to determine the effect of Lactobacillus acidophilus DSM 20079 as a probiotic strain on the adhesion of selected streptococcal strains on the surfaces. The sample comprised 40 isolates of oral streptococci from dental plaque and caries of volunteer persons. Streptococcus mutans ATCC35668 (no24) was as an standard strain.

Lactobacillus Acidophilus-Derived Biosurfactant Effect on GTFB and GTFC Expression Level in Streptococcus Mutans Biofilm Cells.

Streptococcus mutans (S. mutans), harboring biofilm formation, considered as a main aetiological factor of dental caries. Gtf genes play an important role in S.