Publications by authors named "Aref'eva L"

Aim: Study sensitivity of nosocomial microbes--causative agents of post-operative infectious complications in surgical profile patients to bacteriophages.

Materials And Methods: Microbe isolates from biosubstrates of 223 surgical patients served as material. Microbes were isolated from blood, urine, wounds, intravascular catheters, abdominal cavity drainage, discharge of pharynx, trachea (343 strains).

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The morphology of female reproductive organs and anatomy, ultrastructure, and ultrasculpture of seed integuments were studied in more than 150 species of Podocarpales, Cephalotaxales, and Taxales. The main trends of morphological-evolutionary transformations of female fructifications were established in three studied groups of gymnosperms. The taxonomic weight of structural features of the female reproductive organs of Podocarpales, Cephalotaxales, and Taxales was determined.

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The patterns of protein biosynthesis in the embryo and endosperm during embryogenesis in the Scots pine were studied using electrophoresis and biochemistry methods. Proteins of the albumin-globulin fraction were visualized already at the early embryonic stages. The main polypeptide components (48-60, 37-39, and 20-22 kDa) were gradually accumulated in the course of maturation.

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The phylogenetic relationships of gymnospermous and angiospermous plants were studied. To this end, 13 antisera to seed proteins of plant taxa representing all the four classes of the gymnosperms were obtained. The antigens used in immunochemical reactions with these antisera included the proteins of 134 seed samples representing 91 families from all the 11 subclasses of dicotyledons and 64 seed samples representing 33 families from five out of six classes of monocotyledons (according to Takhtajan, 1996).

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Wheat prolamines were preincorporated into the endosperm of maize seed, and concentrations of free and bound amino acids were measured in the endosperm and seedlings during seed germination. Incorporation of foreign prolamines lowered the decrease rate of free proline concentration in both the endosperm (sprouting and shoots) and the embryo (sprouting). The seedlings at the stage of shoots were characterized by higher concentrations of aspartic acid, glutamic acid, and tyrosine.

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An attempt has been undertaken to evaluate interrelations of gymnospermous and dicotyledonous plants on the basis of immunochemical studies of seed proteins. For this purpose, 12 antisera were raised to proteins of taxa representing four gymnosperm classes: Ginkgoopsida, Cycadopsida, Coniferopsida, and Gnetopsida. Seed proteins of eight dicotyledonous subclasses (after Takhtadzhyan, 1987) were used.

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Amino acid composition of dicotyledonous seeds of 82 genera, 67 families, 48 orders, and 8 subclasses (after Takhtadzhyan, 1987). Within the frames of hypothesis of amino acid composition of the least specialized hypothetical taxon we have established a universal trend for all dicotyledonous classes--increased proportion of glutamic acid and arginine. Divergent processes are pronounced in the evolution of amino acid composition within the families and orders.

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Amino acid composition of prolamins and the whole seed in representatives of 52 cereal genera and 22 tribes, as well as amino acid composition of seed protein fractions in representatives of 9 cereal genera are presented. In terms of proposed directed evolution of the seed proteins and adaptive role of prolamins in evolution and distribution of cereals, generalized data on their content in seed protein complex, electrophoretic, immunochemical, and amino acid composition of the seeds, we develop the notion of prolamins biochemical specialization and their polyphyletic origin during evolution of cereal seed protein complex. Seven adaptive types of prolamins are recognized: Sasa, Molinia, Chloris, Zingeria, Poa, Triticum, and Panicum types.

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The authors examined 9 children aged from 13 months to 9 years who, while being in a general hospital, contracted enteroviral encephalitis induced by Coxsackie B3 virus with a possible involvement of Coxsackie B1 virus. This report on a group hospital outbreak of enteroviral encephalitis appears to be the first of the kind in the available literature. The authors discuss the possibility of enteroviral encephalitis and Economo's encephalitis being identical in nature.

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