[The phasic contraction of the guinea pig vas deferens in organotypic culture during periodic stretching].

Vas deferens explants from the adult guinea pigs were cultivated under organotypic conditions with repeated stretching. In control explants with no stretching over 9 days the occurring hypersensitivity is similar to the denervated one. Simultaneously phase component contractile response decreased in a 5.

[The hypersensitivity of the guinea pig vas deferens in organotypic culture under static stretching].

The explants of the guinea pig vas deferens acquired the same hypersensitivity as the denervated ones in cultivation under organotypic conditions. The hypersensitivity reached its maximum in 9 days. The explants cultivated with static stretching during 9 and 15 days preserved the contractile properties of freshly isolated stria.

[A flow laser analyzer of erythrocyte deformability].

The analyser of erythrocyte deformation has been worked out, it allows one to obtain the distribution of the cells according to the deformation degree. The analyser consists of optic electronic block, sample insert unit, signal transformation unit and the information processing unit. The analyser is based on the principle of the small angle light dispersion of laser radiation registration made on each erythrocyte of the population, which was extended due to the influence of the shift efforts when the cell is passing through the narrow measuring capillary.

[Toxicologic evaluation of individual chemical compounds and complex mixtures using a motile cell test object].

The possibility of quantitative prognosis of chemical compounds and complex mixtures toxicity by means of a biotechnical system with suspension of bull spermatozoa sensor was investigated. The concentration of some chemical compounds, inhibiting by half spermatozoa motility has been experimentally determined. A correlation between the values determined and acute toxicity of the compounds tested has been established.

[Laser mass-spectrometric medical microanalysis].

Quantitative analyses of the whole microcomposition at a time without using the standards are shown to be realizable by the laser mass-spectrometry carried out on the domestic device EMAL-2. The techniques of sample preparation are simple and may be applied to analyze the wide range of medical and biological specimens. From the obtained results it is obvious that the mass-spectrometric method is useful in microanalyzing element compositions of medical and biological specimens.

[Changes in the rate of surface blood microcirculation studied by photon-correlation spectroscopy in the practical treatment and diagnosis of diabetes mellitus].

The paper is concerned with the results of studies on the basal blood flow and responses of the microcirculatory channel to physiological and pharmacological loads in health and diabetes mellitus using the method of photon-correlation spectroscopy in the light fiber variant. The rate of the basal blood flow in patients with diabetes mellitus (irrespective of the diabetes type) as compared to that in health was significantly lower in the finger tip and tended toward reduction in the inner side of the forearm, lobule of the auricle and in the conjunctiva. The presence of retinopathies in patients with diabetes mellitus resulted in a significant decrease in the rate of the blood flow in the conjunctiva as compared to those in health and diabetes mellitus without retinopathy.

[Laser flow-type counter-analyzer for microparticles].

A flow-type instrument for studying microparticles distributed in liquid suspensions is presented. With the instrument, one can determine concentrations of microparticles, their size distribution, perform computer-based data analysis. Considerations are also given to the main units of the instrument, their characteristics, and data acquisition and processing.

[Method of determining the activity of cell suspensions as determined by their natural motility].

A new method and setup for determination of motile cell suspension activity are offered. A possibility of measuring the activity with regard to the quota of motile cells and their mean velocity has been shown. Bovine sperm in two diluents was applied as a test object.

[Evaluation of the photon correlation method of measuring skin blood flow and its use in the diagnosis of microcirculation disorders in patients with diabetes mellitus].

A new photon-correlation method for measuring blood flow and traditional methods for blood flow measurements according to the clearance of 133Xe were studied and compared. The measurement data of the skin circulation by the photon-correlation method and according to the clearance of 133Xe are in linear relationship, the correlation ratio being equal to +0.93 (P less than 0.

[Light-guide photon-correlation method of measuring the velocity of blood microcirculation].

The photon correlation method of measuring the velocity of blood microcirculation by using a light guide is described. From the traditional methods it features the high accuracy and responsiveness.

[Study of the kinetics of antigen-antibody reaction by light scattering].

Laser light scattering in conjunction with measurement of the spectral width and integral intensity of light scattering was applied to studying the process of complex formation of antigens and antibodies. The system of polysaccharide group A streptococcus and antibody against it was examined under varying polysaccharide concentrations. The measurements were performed every 10 s for 70 min after combining polysaccharide and antibody solutions.

[Technic of optical mixing spectroscopy in the diagnosis of blood microcirculations].

Optical mixing spectroscopy was used to measure the correlation function of helium-neon laser light (wavelength 0.633 micron) scattered in the finger skin. The mean blood flow velocity was evaluated from the data obtained.

[Influenza virus hemagglutinin: hydrodynamic properties and molecular weight].

Hydrodynamic properties of hemagglutinin isolated with bromelain from influenza virus were studied. The sedimentation constant determined by the method of analytical ultracentrifugation was 9.17 +/- 0.

[Dispersion analysis of viral suspensions by the technic of optical mixing spectroscopy].

To evaluate the heterogeneity of virus populations and determinations of virus monoparticle size in suspensions, a dispersion analysis by the method of spectroscopy of optic mixing was developed. The parameters of influenza USSR/Khabarovsk and parainfluenza Sendai virus suspensions were measured in correlation spectrometer and the results were computerized.