[Remote results of surgical treatment of the patients with multilevel atherosclerotic damage of the main arteries of the lower extremity].

The results of treatment of 131 patients, suffering multilevel atherosclerotic affection of abdominal aorta and the main arteries of the lower extremity, are adduced. Algorhythm of arterial reconstruction performance depending on the spread of the aorto-iliac and femoro-politeal segments affection, was elaborated. There were applied two methods of treatment: reconstruction of aorto-iliac segment only and simultaneous reconstruction of arteries on two levels.

[Application of minimally invasive lumbar sympathectomy in the treatment of patients with obliterating diseases of the lower extremity vessels].

The method of performance and immediate results of miniinvasive lumbar sympathectomy using retroperitoneal miniaccess were adduced in the work. In 2005 yr on the base of division of vascular surgery were operated 19 patients. Application of endoscopic instruments and optic system of illumination gave the possibility to remove lumbar sympathetic nodes in conditions of narrow (3-5 cm) surgical access and operative field.

[Interrelation between protein synthesis and nucleo-cytoplasmic transport of messenger RNA in cells of rat livers in different stages of regeneration].

Synthesis of nonribosomal RNA was increased 2-2.5-fold in nuclei of rat liver cells at early steps of regeneration /2 days after partial hepatectomy/under conditions of protein synthesis inhibition by means of cycloheximide within 3 hrs. Amount of mRNA, transferred from nuclei into cytoplasm, was also 2-fold higher under these conditions as compared with control cells.

[Changes in the mechanisms controlling mRNA stability during the process of liver regeneration in rats].

If the protein biosynthesis was inhibited by cycloheximide, an elevated degradation of total mRNA, including mRNA of membrane-bound and free polyribosomes, occurred in the liver cells at early steps of regeneration, within 48 hrs after partial hepatectomy. Simultaneously, distinct increase in activity of alkaline RNAases was observed in cytoplasm as well as of endo-RNAase--in the membrane-bound and free polyribosomes. As shown in experiments with-p-chloromercuribenzoate, the increase in activity of alkaline RNAases in treatment with cycloheximide was due to a decrease in content of short-living proteins, which are inhibitors of RNAases, in cytoplasm of 48 hr regenerating liver cells.

[Nucleo-cytoplasmic mRNA transport in liver rat and hepatoma cells following suppression of protein synthesis by cycloheximide].

Specific radioactivity of uridylic nucleotide pool was found to be 2-2.5-fold higher in rat liver cells treated with cycloheximide within 3 hrs as compared with the control liver cells. Cycloheximide did not affect the specific radioactivity of the nucleotide pool in cells of Zajhdel ascites hepatoma.

[Factors increasing the effectiveness of Bacillus subtilis fusion].

The factors potentiating the efficacy of fusion of protoplasts of Bacillus subtilis were studied. A 50-fold increase in the yield of primary prototrophs was provided by 90-minute preincubation of protoplasts in a liquid hyperbaric medium and 10-minute treatment with trypsin. Increased efficacy of fusion of protoplasts depended on their regeneration percentage.

[Change in RNase activity in rat liver cells in cycloheximide protein synthesis inhibition].

Administration of cycloheximide to rats does not change the activity of acid and alkaline RNAases in the cytoplasm of hepatocytes. At the same time cycloheximide-induced inhibition of protein synthesis leads to a fall in the activity of RNAase of microsomes and membrane-bound polyribosomes. The activity of RNAase of free polyribosomes of hepatocytes rises considerably under the action of cycloheximide.

[mRNA breakdown in tumor cells in vivo under cycloheximide protein synthesis inhibition].

Amoung of prelabelled mRNA was unaltered in Zajhdel ascites hepatoma of rat cells within 3.5-4 hrs under conditions of treatment with actinomycin D. Due to combined effect of actinomycin D and cycloheximide the content of mRNA in the hepatoma cells was rapidly decreased.

[Selection of a method and surgical tactics in late repeated operations on the lungs and pleura].

The experience with the surgical treatment of 97 patients subjected to late repeated operations on the lungs and pleura is generalized in this work. 68% of these patients had pleural empyema and bronchial fistulae. The authors prefer the radical surgery to pulmonectomy, pleuropulmonectomy, decortication, lung resection and repeated amputation of the main bronchus.

[Stabilization of polyribosomal mRNA in rat liver cells under protein synthesis inhibition by cycloheximide].

It was found that in the course of centrifugation of the postmitochondrial fraction from rat liver in the sucrose concentration gradient, mRNA located in the EDTA-resistant structures sedimented together with polyribosomes. The same structures were detected in the polyribosomal fraction of liver cells after injection of ethionine to the animals. Centrifugation of polyribosomes through a layer of 2.

[Metabolism of mRNAs of membrane-bound and free-polyribosomes from rat liver cells during translation and its inhibition].

Metabolism of membrane-bound and free polyribosomal RNAs was studied under conditions of suppressed synthesis of rRNA induced by small doses of actinomycin D. Besides actinomycin D, cycloheximide was used to suppress the protein synthesis. It was demonstrated that administration of cycloheximide and actinomycin D to rats resulted in a stabilization of membrane-bound polysomal mRNA in liver cells.

[Interrelationship between protein synthesis and mRNA metabolism in rat liver cells].

It is demonstrated that RNA isolated from polyribosomes and postmitochondrial fraction of rat liver cells and bound to nitrocellulose filters (Milliport) represent mRNA. RNA taken from the nitrocellulose filters sedimented in sucrose concentration gradient with a wide peak within the range of 18--6S, attaining a maximum at 12S. The (A+U)/(G+C) ratio of this RNA was equal to 1.

[Rate of ribosome movement along messenger RNA in E. coli under normal and inhibited translation].

Considerable decrease of polysome number (22% as compared with 48% in normally grown culture) was observed under methionine starvation of E. coli Hfr (Met-)culture . At the same time the amount of 70S ribosomes increased up to 32%, while it was 2--6% in the control, the content of free ribosome subunits (50S+30S) being stable.

[Messenger RNA metabolism in E. coli under amino acid starvation conditions].

In E. coli Hfr Kavalli (met-) turnover of mRNA was studied in normal state and in methionine deficiency. This strain of E.