Application of a targeted amplicon sequencing panel to screen for insecticide resistance mutations in Anopheles darlingi populations from Brazil.

Large-scale surveillance and informed vector control approaches are urgently needed to ensure that national malaria programs remain effective in reducing transmission and, ultimately, achieving malaria elimination targets. In South America, Anopheles darlingi is the primary malaria vector and is responsible for the majority of Plasmodium species transmission. However, little is known about the molecular markers associated with insecticide resistance in this species.

Basic Science and Pathogenesis.

Background: The apolipoprotein ε4 (ApoE4) allele is a major risk factor for sporadic Alzheimer's disease (AD) and was shown to promote amyloid-β (Aβ) accumulation and mediate pathophysiological processes in AD. Although the molecular interaction between Aβ and ApoE is acknowledged, the precise nature of this interaction remains unclear. This study aims to explore the biophysical and biochemical nature of the interaction between Aβ and ApoE in the ε3 and ε4 isoforms.

High throughput phenotyping in soybean breeding using RGB image vegetation indices based on drone.

This study investigates the effectiveness of high-throughput phenotyping (HTP) using RGB images from unmanned aerial vehicles (UAVs) to assess vegetation indices (VIs) in different soybean pure lines. The VIs were accessed at various stages of crop development and correlated with agronomic performance traits. The field research was conducted in the experimental area of the Mato Grosso do Sul Foundation, Brazil, with 60 soybean pure lines.

XbaI polymorphism in the APOB gene and its association with increased cholesterol in children and adolescents: Ouro Preto study.

Atherosclerotic vascular changes can begin during childhood, providing risk for cardiovascular disease (CVD) in adulthood. Identifiable risk factors such as dyslipidemia accelerate this process for some children. The apolipoprotein B (APOB) gene could help explain the inter-individual variability in lipid levels among young individuals and identify groups that require greater attention to prevent CVD.

Higher levels of plasmatic saturated fatty acid were significantly associated with liver fibrosis in HIV mono-infection: A case-control study.

Background: The relationship between plasmatic fatty acid (FA) composition and liver fibrosis remains scarce in people living with HIV/AIDS (PLWHA). We aimed to evaluate the association of plasmatic FAs and liver fibrosis in HIV mono-infected individuals.

Methods: This case-control study included PLWHA with liver fibrosis (cases) and randomly selected subjects without fibrosis (controls) from the PROSPEC-HIV study (NCT02542020).

Historical and future heat-related mortality in Portugal's Alentejo region.

Background: The increased severity of extreme weather and anticipated climate change has intensified heat stress-related mortality worldwide. This study examines the historical short-term effects of heat on mortality in Alentejo, Portugal's warmest region, and projects it up to the end of the century.

Methods: Using data from 1980 to 2015 during warm seasons (May-September), the association between daily mortality by all-causes and mean temperature was examined following a case time series design, applied at both regional and subregional scales.

Vaccination coverage according to race or skin color in children born in 2017-2018 in Natal, Rio Grande do Norte, Brazil: a population survey.

Objective: To analyze vaccination coverage up to 24 months of age according to race/ skin color in the 2017-2018 live birth cohort in Natal, Rio Grande do Norte, Brazil.

Methods: Population-based survey conducted in 2020 and 2021. Vaccination coverage up to 24 months of age was estimated according to administered, valid and timely doses.

Neutrophil/lymphocyte and platelet/lymphocyte ratio in seropositive women for human immunodeficiency virus (HIV) and human papillomavirus (HPV) coinfection.

This study aims to investigate the possible association between neutrophil/lymphocyte and platelet/lymphocyte ratio in women with HIV, undergoing antiretroviral treatment, with HPV coinfection. This is a cross-sectional study with HIV positive women; their biological samples were collected for laboratory tests (complete blood count) and oncotic cytology for detection of HPV DNA, by PCR-Nested (PGMY and GP primers). Viral load and CD4 and CD8 T-cells counts were obtained from medical records.

Effect of the consumption of cashew nut (Anacardium occidentale l.) flour-based biscuits in overweight children: a pilot randomized clinical trial.

Background: cashew nut is an almond known for its cardiovascular benefits in adults and weight gain effects in malnourished children, as supported by research. However, its impact on overweight children remains unexplored.

Objective: to analyze the effect of consuming biscuits made with cashew nut flour on the blood glucose and serum triglyceride levels of children with overweight/obesity over four weeks.

Omega-3 Fatty Acids Weaken Lymphocyte Inflammatory Features and Improve Glycemic Control in Nonobese Diabetic Goto-Kakizaki Rats.

Background/objectives: Goto-Kakizaki (GK) rats exhibit insulin resistance and type 2 diabetes mellitus (T2DM) without obesity. This study explored the effects of ω-3 fatty acid supplementation on T lymphocyte polarization in Wistar (WT) and GK rats.

Methods: They were administered ω-3 fatty acid-rich fish oil (FO) containing eicosapentaenoic (540 mg/g) and docosahexaenoic acids (100 mg/g) by oral gavage at 2 g/kg, thrice a week for 8 weeks.

Recurrent neurogenic shock as a rare postoperative complication associated with pancreaticoduodenectomy with mesopancreas excision in elderly patient: a case report.

Background: Surgical resection of locally advanced or borderline pancreatic ductal adenocarcinoma is a recognized procedure with curative intent performed in specialized oncology centers. Postoperative dysautonomia such as gastroparesis, mild hypotension, and diarrhea are common in elderly patients undergoing pancreaticoduodenectomy. A distinctive feature of our case, is the severing of an important sympathetic chain by the surgical procedure, leading to recurrent severe neurogenic shock.

Serum metalloproteinase-7 as a biomarker of progressive pulmonary fibrosis.

Introduction: Progressive pulmonary fibrosis (PPF) corresponds to any fibrotic interstitial lung disease (ILD) other than idiopathic pulmonary fibrosis (IPF) that presents clinical, physiological and/or radiological evidence of disease progression similar to IPF. Matrix metalloproteinases (MMPs) have been implicated in the pathogenesis of pulmonary fibrosis and are associated with disease progression and reduced survival in IPF and other fibrotic ILDs. This study aimed to investigate the role of serum levels of MMP-1 and MMP-7 in patients with fibrotic non-IPF ILD as possible biomarkers of patients at risk of developing PPF.

Lessons learned from the risk-informed urban development initiative in the SADC region.

Unlabelled: This study presents the systemisation of lessons learned from the urban sector in which the measures based on the guiding principle of risk-informed development (RID) have been implemented in the Southern African Development Community (SADC) region at the national and/or sub-national levels. Despite notable risks in the region, these are not adequately considered in urban development planning and programming. Aiming at strengthening RID in the SADC region, the objectives of this peer-to-peer exchange were achieved through virtual workshops, roundtables and briefings on a cloud-based and open-source BigBlueButton Web conferencing system.

PANCREATODUODENECTOMY AS TREATMENT FOR RECURRENT ACUTE PANCREATITIS DUE TO PANCREAS DIVISUM.

Background: Pancreas divisum is an anatomical abnormality where the junction of the main and accessory pancreatic duct fails to occur and the smaller-caliber duct acts as dominant, resulting in overload during the drainage of the organ's secretion through the minor duodenal papilla.

Aims: To report a case of recurrent acute pancreatitis due to symptomatic pancreas divisum who underwent pancreatoduodenectomy.

Case Report: A 21-year-old male patient presented with intermittent painful crises, located in the upper abdomen, with radiation to the back, associated with nausea and vomiting, for the past three years.

Transition to family parenting in the face of the first child: a scoping review.

Objectives: to identify and summarize the elements that characterize the family transition process in relation to the first child.

Methods: a scoping review was carried out based on JBI methodology, in six databases, following the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews checklist.

Results: ten articles were included with factors characterizing the transition, such as hindering/facilitating conditions that influence the process, important support structures in adaptation and strategies/responses used in the transition process.

Concept analysis of youth: understanding the plurality of subjects.

Objective: To analyze the concept of "youth."

Methods: This is a concept analysis guided by the method proposed by Walker and Avant, operationalized through an integrative literature review. The search in scientific databases was carried out using the descriptors: youth; young; adolescence.

Probiotication of Plum Pulp and Conditions Effects Freeze-Drying in Cell Viability, Functional Properties and Antioxidant Activity.

This study investigated the effects of fermenting plum pulp with ssp. (BAL) on its physicochemical and bioactive properties, as well as the optimization of the freeze-drying (FD) process to develop a fruit-based probiotic delivery system. Fermentation significantly reduced the pH and total acidity of the pulp, achieving a cell viability of 11 log CFU/mL.

Association between chronic health conditions and severe acute respiratory syndrome in pregnant women: an exploratory study.

Objective: To analyze the association between chronic health conditions and severe acute respiratory syndrome in pregnant women.

Method: Retrospective, exploratory study conducted with 1,152 pregnant women from all 27 states of Brazil who sought hospital treatment and were diagnosed with severe acute respiratory syndrome between 2020 and 2022. Public data from the Influenza Epidemiological Surveillance Information System (SIVEP-Gripe) of the Brazilian Ministry of Health were used.

Search for Soft Unclustered Energy Patterns in Proton-Proton Collisions at 13 TeV.

The first search for soft unclustered energy patterns (SUEPs) is performed using an integrated luminosity of 138  fb^{-1} of proton-proton collision data at sqrt[s]=13  TeV, collected in 2016-2018 by the CMS detector at the LHC. Such SUEPs are predicted by hidden valley models with a new, confining force with a large 't Hooft coupling. In events with boosted topologies, selected by high-threshold hadronic triggers, the multiplicity and sphericity of clustered tracks are used to reject the background from standard model quantum chromodynamics.