Feasibility of Flow Cytometry Analysis of Gastrointestinal Tract-Residing Lymphocytes in Hematopoietic Stem Cell Transplant Recipients.

The feasibility of lymphocyte isolation and flow cytometry using a single endoscopic biopsy specimen from the gastrointestinal tract of patients who have undergone hematopoietic stem cell transplantation has not been investigated. We acquired 51 endoscopic biopsy specimens from the gastrointestinal tract of 35 patients. We divided the flow cytometry samples into two groups: group A, successful lymphocyte isolation (n=24), and group B, incomplete isolation (n=27).

Increased CCR4+ and Decreased Central Memory CD4+ T Lymphocytes in the Background Gastric Mucosa of Patients Developing Gastric Cancer After Helicobacter pylori Eradication: An Exploratory Study.

The composition of lymphocytes in the gastric mucosa following the eradication of () in patients with and without gastric cancer has not been compared. This study performed a single spot analysis of gastric mucosal lymphocytes after eradication in patients with (n = 13) and without (n = 20) gastric cancer. Our comprehensive analysis of lymphocyte composition in the gastric mucosa revealed that: i) the proportion of CD8/CD3 cells was relatively higher in the peri-tumor mucosa than in the background mucosa; ii) the proportion of CCR4/CD3 cells was higher, and the ratio of CD62L/CD3CD4 cells was relatively lower in the gastric mucosa of cancer patients than in non-cancer patients; and iii) the proportion of CD45RACD62L/CD3CD4 cells, namely, the central memory CD4 T-cell fraction, was lower in the gastric mucosa of cancer patients than in non-cancer patients.