Publications by Araceli Flores-Sanchez

Publications by authors named "Araceli Flores-Sanchez"

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High amounts of medium-chain-length polyhydroxyalkanoates subunits can be accumulated in recombinant Cupriavidus necator with wild-type synthase.

Flores-Sánchez Araceli Ramsay Juliana A Vergara-Porras Berenice Pérez-Guevara Fermin Ramsay Bruce A

J Biotechnol

April 2022

Article Synopsis

- Recombinant Cupriavidus necator H16/pMPJAS03 can create short/medium-chain-length (scl-mcl) PHA copolymers using a native enzyme and was grown on fructose combined with canola oil.
- The cells produced a polymer primarily made up of 3-hydroxybutyrate (3HB) and significantly higher amounts of 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD) compared to previous yields.
- Increasing the decanoic acid in the feed enhanced the production of 3HD and impacted the properties of the resulting polymers, demonstrating the potential for optimizing enzyme combinations to produce

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Biosynthesis of polyhydroxyalkanoates from vegetable oil under the co-expression of fadE and phaJ genes in Cupriavidus necator.

Araceli Flores-Sánchez Arthi Rathinasabapathy Ma Del Rocío López-Cuellar Berenice Vergara-Porras Fermín Pérez-Guevara

Int J Biol Macromol

December 2020

The acyl-CoA dehydrogenase (FadE) and (R)-specific enoyl-CoA hydratase (PhaJ) are functionally related to the degradation of fatty acids and the synthesis of polyhydroxyalkanoates (PHAs). To verify this, a recombinant Cupriavidus necator H16 harboring the plasmid -pMPJAS03- with fadE from Escherichia coli strain K12 and phaJ1 from Pseudomonas putida strain KT2440 under the arabinose promoter (araC-P) was constructed. The impact of co-expressing fadE and phaJ genes on C.

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