Publications by authors named "Aqing Yao"
Systems for introducing DNA genes-of-interest into mammalian cellular genomes have ranged from the use of different physical techniques to viruses including retroviruses. We have developed a microinjection method for an efficient and permanent integration of a DNA transgene into the cell genome by use of the retrovirus integrase. A 3.
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- Human mesenchymal stem cells (hMSCs) can differentiate into various tissue types, making them promising for cell and gene therapies.
- The study demonstrated that glass needle-mediated microinjection effectively delivered macromolecules like DNA to hMSCs, achieving a 76% cell viability post-injection.
- Microinjection of supercoiled DNA vectors resulted in significantly higher gene expression and the formation of long-lasting GFP-positive colonies, indicating potential for future genetic modifications in hMSCs.