Publications by authors named "Apurva Bhasin"

The Virus BioResistor (VBR) is a biosensor capable of rapid and sensitive detection of small protein disease markers using a simple dip-and-read modality. For example, the bladder cancer-associated protein DJ-1 (22 kDa) can be detected in human urine within 1.0 min with a limit of detection (LOD) of 10 pM.

View Article and Find Full Text PDF

The 2018 Nobel Prize in Chemistry recognized in vitro evolution, including the development by George Smith and Gregory Winter of phage display, a technology for engineering the functional capabilities of antibodies into viruses. Such bacteriophages solve inherent problems with antibodies, including their high cost, thermal lability, and propensity to aggregate. While phage display accelerated the discovery of peptide and protein motifs for recognition and binding to proteins in a variety of applications, the development of biosensors using intact phage particles was largely unexplored in the early 2000s.

View Article and Find Full Text PDF

DJ-1, a 20.7 kDa protein, is overexpressed in people who have bladder cancer (BC). Its elevated concentration in urine allows it to serve as a marker for BC.

View Article and Find Full Text PDF

The virus bioresistor (VBR) is a chemiresistor that directly transfers information from virus particles to an electrical circuit. Specifically, the VBR enables the label-free detection of a target protein that is recognized and bound by filamentous M13 virus particles, each with dimensions of 6 nm ( w) × 1 μm ( l), entrained in an ultrathin (∼250 nm) composite virus-polymer resistor. Signal produced by the specific binding of virus to target molecules is monitored using the electrical impedance of the VBR: The VBR presents a complex impedance that is modeled by an equivalent circuit containing just three circuit elements: a solution resistance ( R), a channel resistance ( R), and an interfacial capacitance ( C).

View Article and Find Full Text PDF

Synopsis of recent research by authors named "Apurva Bhasin"

  • - Apurva Bhasin's research focuses on the development and enhancement of the Virus BioResistor (VBR), a biosensor designed for quick and sensitive detection of disease markers, particularly in urine samples, such as the bladder cancer-associated protein DJ-1.
  • - Recent studies led by Bhasin have demonstrated the VBR's capability to detect low concentrations of proteins (as low as 10 pM) in a rapid manner (within one minute), showcasing its potential for clinical diagnostics.
  • - The research also explores innovative uses of bacteriophages, benefiting from phage display technology to overcome common issues encountered with traditional antibodies, thus opening new avenues for biosensor applications.

A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_session9fc5o8v8fs4jkach15p2qmsid683fi96): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once