Publications by authors named "Apuleyo Yanez"
Contractility and Calcium Transient Maturation in the Human iPSC-Derived Cardiac Microfibers.
ACS Appl Mater Interfaces
August 2022
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are considered immature in the sarcomere organization, contractile machinery, calcium transient, and transcriptome profile, which prevent them from further applications in modeling and studying cardiac development and disease. To improve the maturity of hiPSC-CMs, here, we engineered the hiPSC-CMs into cardiac microfibers (iCMFs) by a stencil-based micropatterning method, which enables the hiPSC-CMs to be aligned in an end-to-end connection for prolonged culture on the hydrogel of physiological stiffness. A series of characterization approaches were performed to evaluate the maturation in iCMFs on both structural and functional levels, including immunohistochemistry, calcium transient, reverse-transcription quantitative PCR, cardiac contractility, and electrical pacing analysis.
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- 3D cardiac engineered constructs are advancing cardiac regenerative medicine by enabling better modeling of both normal and diseased heart tissues, moving beyond basic treatments like heart transplants.
- The review discusses different cell types in cardiac tissues and their connection to modern fabrication techniques for creating these constructs, which can help understand heart diseases and offer new therapeutic options.
- Future directions focus on developing specialized and personalized constructs that accurately replicate the unique structure and function of specific areas in native cardiac tissues.