Low-temperature effects on docosahexaenoic acid biosynthesis in sp. TIO01 and its proposed underlying mechanism.

Background: species are known for their abundant production of docosahexaenoic acid (DHA). Low temperatures can promote the biosynthesis of polyunsaturated fatty acids (PUFAs) in many species. This study investigates low-temperature effects on DHA biosynthesis in sp.

Segregation but Not Replication of the Chromosome Terminates at .

Coordination between chromosome replication and segregation is essential for equal partitioning of genetic material between daughter cells. In bacteria, this is achieved through the proximity of the origin of replication, , and the chromosome partitioning site, We report here that in , segregation but not replication is also controlled at the terminus region of the chromosome. Using the fluorescent repressor operator system (FROS), we investigated chromosome segregation in strain PAO1-UW, wherein the chromosome dimer resolution site, , is asymmetrically positioned relative to In these cells, segregation proceeded sequentially along the two chromosomal arms and terminated at In contrast, chromosome replication terminated elsewhere, opposite from We further found two large domains on the longer arm of the chromosome, wherein DNA segregated simultaneously.

Pseudomonas aeruginosa Condensins Support Opposite Differentiation States.

Unlabelled: Condensins play a key role in global chromosome packing. Pseudomonas aeruginosa encodes two condensins, SMC-ScpAB and MksBEF. We report here that the two proteins are involved in the differentiation of the bacterium and impose opposite physiological states.

Upconversion nanoparticles: synthesis, surface modification and biological applications.

Unlabelled: New generation fluorophores, also termed upconversion nanoparticles (UCNPs), have the ability to convert near infrared radiations with lower energy into visible radiations with higher energy via a nonlinear optical process. Recently, these UCNPs have evolved as alternative fluorescent labels to traditional fluorophores, showing great potential for imaging and biodetection assays in both in vitro and in vivo applications. UCNPs exhibit unique luminescent properties, including high penetration depth into tissues, low background signals, large Stokes shifts, sharp emission bands, and high resistance to photobleaching, making UCNPs an attractive alternative source for overcoming current limitations in traditional fluorescent probes.