Publications by authors named "Aparna Uppuluri"
Article Synopsis
- Bacterial membranes are influenced by various evolutionary factors, with the enzyme MprF playing a crucial role in modifying membrane lipids.
- MprF synthesizes lysyl-phosphatidylglycerol (Lys-PG) and a new lipid, lysyl-glucosyl-diacylglycerol (Lys-Glc-DAG), prompting further investigation of MprF's substrate specificity in other bacteria.
- Using protein sequence analysis and machine learning, researchers discovered additional MprF products and the presence of diglucosyl-diacylglycerol (Glc-DAG) as a new substrate, highlighting the enzyme’s evolutionary significance across different bacterial species.
Bacterial membranes are complex and dynamic, arising from an array of evolutionary pressures. One enzyme that alters membrane compositions through covalent lipid modification is MprF. We recently identified that MprF synthesizes lysyl-phosphatidylglycerol (Lys-PG) from anionic PG, and a novel cationic lipid, lysyl-glucosyl-diacylglycerol (Lys-Glc-DAG), from neutral glycolipid Glc-DAG.
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