Publications by authors named "Aparecida Perpetuo Silveira-Carvalho"
Evaluation of Serological and Molecular Tests Used for the Identification of Infection in Patients Treated in an Ophthalmology Clinic of a Public Health Service in São Paulo State, Brazil.
Front Cell Infect Microbiol
September 2020
Article Synopsis
- Ocular toxoplasmosis is a common infection that can lead to serious eye problems, particularly prevalent in Brazil.
- The study compared various diagnostic methods (ELISA, ELFA, cPCR, Nested PCR, and qPCR) on patient groups with and without toxoplasmosis-related eye lesions.
- Results showed that while ELISA was more sensitive and ELFA more specific for serological diagnosis, qPCR had higher sensitivity but lower specificity than the other molecular methods.
Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil.
Diagn Microbiol Infect Dis
September 2017
Article Synopsis
- - Toxoplasmosis can seriously affect pregnant women, so accurate diagnosis is crucial using various testing methods.
- - A study compared serological tests (ELISA and ELFA) and different PCR techniques (cPCR, Nested PCR, qPCR) in pregnant women, both with and without symptoms of the disease.
- - The study found that while ELISA and ELFA showed similar results, ELFA detected more positive cases; however, molecular methods like cPCR had better sensitivity but lower specificity compared to others.
Anti-Toxoplasma gondii antibodies in patients with beta-hemoglobinopathies: the first report in the Americas.
BMC Res Notes
June 2017
Article Synopsis
- This study is the first to investigate Toxoplasma gondii infection in Brazilian patients with sickle cell anemia and severe beta-thalassemia, evaluating a total of 158 individuals.
- The results showed variations in anti-T. gondii IgG antibody presence, with 43.5% in sickle cell patients, 18.1% in homozygous beta-thalassemia patients, and 50% in heterozygous beta-thalassemia patients, but no significant gender differences in infection rates.
- The study concluded that younger sickle cell anemia patients had a potentially lower risk of T. gondii infection, highlighting the need for further research in this area.
The objective of this study was to investigate the influence of the genes encoding the KIR receptors and their HLA ligands in the susceptibility of ocular toxoplasmosis. A total of 297 patients serologically-diagnosed with toxoplasmosis were selected and stratified according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis. The group of patients with scars/lesions was further subdivided into two groups according to the type of ocular manifestation observed: primary (n = 120) or recurrent (n = 28).
View Article and Find Full Text PDFFrequency of anti- Toxoplasma gondii IgA, IgM, and IgG antibodies in high-risk pregnancies, in Brazil.
Rev Soc Bras Med Trop
January 2017
Introduction: Toxoplasmosis during pregnancy can be severe; thus, it is essential to diagnose the disease via serological tests.
Methods: An enzyme-linked immunosorbent assay (ELISA) was used to investigate anti-Toxoplasma gondii immunoglobulin A (IgA), M (IgM) and G (IgG) antibodies in 62 high-risk pregnant women.
Results: Forty-three (69.
MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis.
PLoS One
June 2016
This study investigated whether polymorphisms of the MICA (major histocompatibility complex class I chain-related gene A) gene are associated with eye lesions due to Toxoplasma gondii infection in a group of immunocompetent patients from southeastern Brazil. The study enrolled 297 patients with serological diagnosis of toxoplasmosis. Participants were classified into two distinct groups after conducting fundoscopic exams according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis.
View Article and Find Full Text PDFBackground: Toxoplasmosis was recently included as a neglected disease by the Center for Disease Control. Ocular toxoplasmosis is one clinical presentation of congenital or acquired infection. The laboratory diagnosis is being used worldwide to support the clinical diagnosis and imaging.
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